Background: RADA16I represents one of promising hydrogel forming peptides. Several implementations of RADA16I hydrogels have proven successful in the field of regenerative medicine and tissue engineering. However, RADA16I peptides used in various studies utilize synthetic peptides and so far, only two research articles have been published on RADA16I peptide recombinant producti...

Objective(s): Polyethylenimine (PEI) is one of the most widely used polymers in gene delivery. The aim of this study was to modify PEI by replacing some of its primary amines with Brevinin 2R (BR-2R) peptide in order to increase the efficiency of gene delivery.Materials and Methods: Polyethylenimine was modified by BR-2R peptide by two d...

Cancer disease is a great concern in the worldwide public health and current treatments do not give satisfactory results, so, developing novel therapeutic agents to combat cancer is highly demanded. Nowadays, anticancer peptides (ACPs) are becoming promising anticancer drug candidates. This is due to several advantages inherited in peptide molecules, such as being usually with small size, high ...

Cancer disease is a great concern in the worldwide public health and current treatments do not give satisfactory results, so, developing novel therapeutic agents to combat cancer is highly demanded. Nowadays, anticancer peptides (ACPs) are becoming promising anticancer drug candidates. This is due to several advantages inherited in peptide molecules, such as being usually with small size, high ...

Objective(s):Antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. The aim of this study was to produce polyclonal antibody against a synthetic peptide derived from N-terminal region of β-actin protein to be used as a protein loading control in western ...

Background: Deoxyribozymes (Dzs) can play a role as gene expression inhibitors at mRNA level. Among Dzs, the 10-23 deoxyribozyme has significant potentials for treatment of diseases. Designed Dz includes a catalytic core made of 15 deoxyribonucleotides and two binding arms consisted of 6-12 nucleotides for site specific binding to target RNA and hydrolysis. The enzyme has characteristic feature...

background: eukaryotic proteins generally have signal peptides, which are not only crucial for their secretion efficiencies but are important for their expression levels. the coagulation factor ix (fix) is a glycoprotein that plays a fundamental role in a blood coagulation pathway. reduced levels or dysfunctional fix are associated with hemophilia b. to improve the hfix secretion efficiency in ...

introduction: glycoprotein b (gb) is the major antigen for induction of humoral responses against human cytomegalovirus (hcmv) making it an attractive candidate for immune prophylaxis. in the present study, the humoral immune response of balb/c mice to a truncated hcmv gb protein fused with gfp was evaluated. methods: the truncated gb coding sequence was synthesized and cloned in pegfpn1 eukary...

background: type 2 diabetes (t2d) is a chronic metabolic disorder in which beta-cells are destroyed. the islet amyloid polypeptide (iapp) produced by beta-cells has been reported to influence beta-cell destruction. objective: to evaluate if iapp can act as an autoantigen and therefore, to see if cd8 + t-cells specific for this protein might be present in t2d patients. methods: peripheral blood ...

Background: Biological molecules such as therapeutic proteins and peptides have provided ample opportunities for further improvements in health wellbeing. However, biomacromolecules face stability challenges regarding premature processing digestion, whenever any enteric delivery route is considered. Hence, oral guar films entrapping peptide-containing chitosan microparticles been developed a ne...