Hexokinase-deficient mutants and wild-type Chinese-hamster ovary cells have been used to investigate the role of hexokinase in uptake and accumulation of 2-D-deoxyglucose (2-dGlc). The evidence for a specific sugar transport system in both types of cells is that there is similar saturable phloretin-sensitive uptake of 2-dGlc and 3-O-methyl-D-glucose (3-OMG) in both types of cell. In wild-type c...

Acetaminophen, p-aminophenol, and oxyphenbutazone interfere with the glucose oxidase/peroxidase method for glucose. Structurally related compounds that lack a free phenolic hydroxyl group (acetanilide, aniline, and phenylbutazone) do not interfere. During the analytical procedure acetaminophen is consumed. One mole of acetaminophen leads to an apparent loss of four moles of glucose. The hexokin...

Kinetic studies of the glucose-dependent monomer-dimer reaction of yeast hexokinase PI at pH 8.0 in the presence of 0.1 M-KCl have been carried out using the fluorescence temperature-jump technique. A slow-relaxation effect was observed which was attributed from its dependence on enzyme concentration to the monomer-dimer reaction; the reciprocal relaxation times tau-1 varied from 3 s-1 at low c...

Concentrations of hexokinase in 18 types of transplanted rat tumors were significantly correlated with the measured growth rates and also paralleled the degrees of loss of histological differentiation of these tumors. The correlations confirm the similar relationship already found among hepatomas and extends it to some nonhepatic tumors of the rat. The normal tissues, fetal and regenerating liv...

Tigogenin Glucose-6-phosphatase Hexokinase Glycogen

Rapid and accurate measurement of capillary glucose level using point-of-care testing (POCT) is needed to maintain the patient’s normoglycemic status in obtaining adequate management. The POCT method should be evaluated determine analytical performance by comparing with hexokinase as a reference provide reliable results. Objectives: To determined correlation between dehydrogenase-nicotinamide a...

The rabbit VX2 tumor when implanted in the liver has proven convenient as a model for studying hepatocellular carcinomas. However, its metabolic properties have not been well studied. Significantly, studies described here show that the VX2 tumor exhibits a high glycolytic/high hexokinase phenotype that is retained following implantation and growth in rabbit liver. In addition, results of a limi...