Halloysite nanotube supported ruthenium catalyst was prepared by an easy and reproducible in situ bottom up approach. Ruthenium (III) chloride reduced sodium borohydride the presence of halloysite nanotubes dispersed water. Several analyses confirm immobilization particles onto aluminosilicate support. The synthesized then tested hydrogenation 1-hexadecene, a highly hydrophobic compound, aqueou...

In this article, the novel inventive steps for the extraction and quantification of sulfated glycosaminoglycan (GAG) from Acanthaster planci starfish, generally known as crown-of-thorns (COT), are reported. Starfish have been implicated with collagenous distributions within their body anatomy, thus making it a prima facie fact searching for the possibility that GAGs can be isolated from COT. In...

Human cationic and anionic trypsins are sulfated on Tyr154, a residue which helps to shape the prime side substrate-binding subsites. Here, we used phage display technology to assess the significance of tyrosine sulfation for the specificity of human trypsins. The prime side residues P1'-P4' in the binding loop of bovine pancreatic trypsin inhibitor (BPTI) were fully randomized and tight bindin...

We investigated the mechanisms of anticoagulant activity mediated by sulfated galactans. The anticoagulant activity of sulfated polysaccharides is achieved mainly through potentiation of plasma cofactors, which are the natural inhibitors of coagulation proteases. Our results indicated the following. 1) Structural requirements for the interaction of sulfated galactans with coagulation inhibitors...

Inhibitors of tumor angiogenesis and metastasis are rapidly emerging as important new drug candidates for cancer therapy. To facilitate the identification of such drugs, we recently developed novel and rapid in vitro assays for human angiogenesis and for the extracellular matrix-degrading enzyme heparanase, which has been implicated in tumor metastasis. In this study, sulfated oligosaccharides,...

The aggregation status of chlorophyll a (Chl a) and the ability of four cyclodextrins, hydroxypropyl-beta-cyclodextrin (HP-beta-CD), hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD), heptakis(2,6-di-O-methyl)-beta-cyclodextrin (DIMEB), and heptakis(2,3,6-tri-O-methyl)-beta-cyclodextrin (TRIMEB), to solubilize the pigment in the complete cellular medium RPMI 1640 was estimated by means of UV-Vis a...

Cyclodextrins (CDs) are a family of cyclic oligosaccharides composed ofα-(1, 4) linked glucopyranose subunits. CDs have been found as potential candidates because of their ability to alter physical, chemical and biological properties of guest molecules through the formation of inclusion complexes. Pure cyclodextrins that were suitable for pharmaceutical applications did not come available until...

Per-O-methylated cyclodextrins containing a single 2-O-(2-acetate), 2-O-(3-propanoate) or a 6-carboxylate were investigated for glycosidase activity on p-nitrophenyl glycosides. The former two compounds displayed enzyme catalysis giving rate accelerations of 500-1000, while the latter compound gave marginal catalysis. These results show that per-O-methylated cyclodextrins direct substrate bindi...

The electrochemical behavior of the corrosion resistance AISI 304 embedded in concrete manufactured as indicated by ACI 211.1 method was evaluated. specimens were exposed for more than 150 days to highly aggressive marine-sulfated environment, solution with a concentration 5% NaCl and Na2SO4. technique Resistance Linear Polarization (Rp) used determine rate (Icorr) monitoring potential (Ecorr)....

Methods currently in use for the quantitative measurement of sulfated serum bile acids usually lead to low recoveries. The present technique uses two internal standards and involves enzymatic hydrolysis followed by extraction of the non-sulfated fraction in ether at pH 4.6. The sulfated fraction is then solvolysed before gas-liquid chromatographic analysis of the individual bile acids of both f...