Multiple forms of occludin were found in Madin-Darby canine kidney (MDCK) cells. In the absence of cell-to-cell contacts, achieved by incubating cells in low-calcium growth medium, a cluster of lower-molecular-weight (LMW) occludin bands (∼65,000-68,000) was present in both MDCK I and II cells. On formation of tight junctions, achieved by changing the low-calcium growth medium to normal-calcium...

Using monoclonal antibodies to distinct determinants of a human high-molecular weight melanoma-associated antigen (HMW-MAA), a double determinant immunoassay has been developed. The assay is specific and reproducible. Its sensitivity is influenced by the incubation time of antibodies with antigen sources and the combination of antibodies, as well as by the pH of the buffer and the incubation ti...

In human serum, low molecular weight (LMW) proteins play an important role in major biological processes which were masked by high molecular weight (HMW) proteins. Separations and identification of LMW proteins of interest from serum is very intricate task. The objective of the present study was to develop in-house acetonitrile (ACN) precipitation method for separation of LMW proteins from high...

High molecular weight glutenin subunits (HMW-GS) play an essential role in wheat processing quality. In this study, we evaluated the genetic pattern with HMW-GS composition between generations and examined whether agronomic and quality traits were correlated with each other. A wheat (Triticum aestivum L.) cultivar with high protein content and 2 cultivars with low protein content were subjected...

The need of natural antimicrobials is paramount to avoid harmful synthetic chemicals. The study aimed to determine the antifungal activity of natural compound chitosan and its nanoparticles forms against Candida albicans, Fusarium solani and Aspergillus niger. Chitosan nanoparticles were prepared from low (LMW), high molecular weight (HMW) chitosan and its derivative, trimethyl chitosan (TMC). ...

Beneficial effects of peroxisome proliferator-activated receptor alpha (PPAR alpha) agonists have been reported in improving insulin sensitivity and raising serum total adiponectin. High molecular weight (HMW) adiponectin, which is secreted from adipocytes, and visfatin, which is also expressed in adipose tissue, is related to glucose metabolism. In view of the additive effects of PPAR alpha ag...

The aim of the study was to examine the association between sexual precocity and high-molecular weight (HMW)-adiponectin and investigate the correlation of insulin resistance and estrogen levels in obese children. In total, 60 obese children (30 boys and 30 girls) with sexual precocity were included in group A, 60 obese children (30 boys and 30 girls) without sexual precocity were included in g...

OBJECTIVES Whereas accumulating evidence indicates close associations between rhinitis and asthma, little is known about the relationships between occupational rhinitis (OR) and occupational asthma (OA). This study analyses the prevalence of OR associated with OA, globally and according to the various causal agents, and investigates the temporal relationships between these two conditions. MET...

The HMW effect in non-commutative quantum mechanics is studied. By solving the Dirac equations on non-commutative (NC) space and non-commutative phase space, we obtain topological HMW phase on NC space and NC phase space respectively, where the additional terms related to the space-space and momentum-momentum non-commutativity are given explicitly. PACS number(s): 02.40.Gh, 11.10.Nx, 03.65.-w

PG-F2 and PG-HMW from Panax ginseng are pectin-type polysaccharides and PG-HMW might be an arabinogalactan. They demonstrated strong anti-adhesive activities against oral and skin pathogens to host cell lines in a dose-dependent manner from 0.1 to 2.0 mg/ml. While enzymatic hydrolysis caused complete loss of anti-adhesive activities, partial hydrolysis produced oligosaccharides with anti-adhesi...