We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.

Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

Fully computerized hospital information systems (HIS) are spreading in Japanese hospitals. Most HIS consist of server computers, terminals, a database, and a LAN. The introduction of HIS has enabled quick communication between doctors and staff and the immediate dissemination of patient information. Installation of a wireless data communication system compatible with a HIS has been reported to ...

introduction: hospital information system (his) is a computerized system which is used as an electronic tool for patient information management and plays a significant role in providing quality health care services. therefore, his users' satisfaction about the quality of the services offered is crucial in assessing and more importantly improving the technical aspects of the system. on the other...

Hospital Information Systems (HIS) play a significant role in providing quality healthcare services. However, HIS lag behind their industrial counterparts in providing quality (i.e., timely, accurate, complete) information and have been the target of many criticisms for alleged shortcomings. The aim of this research is to identify the requirements for HIS to assist in providing quality healthca...