the purification of biomolecules is a necessary step in many biochemical researches. in this regard, developments of convenient, specific and low cost methods of purification are of particular interest. given the human hemoglobin (hb) affinity toward some charged carbohydrates, interaction of this molecule with human chorionic gonadotropin (hcg) which is a glycoprotein hormone containing sialic...

Human pituitary glands contained a form of growth hormone with a molecular weight of 45,000 that did not dissociate in 8 M urea or 1% sodium dodecyl sulfate. The material was isolated by a combination of ion exchange chromatography on DEAE-cellulose in a urea-containing buffer and gel filtration on Sephadex. Upon reduction with 2-mercaptoethanol or oxidation with performic acid prior to electro...

A bovine growth hormone (bGH) analog, bGH-G119R, has been shown to act as a functional antagonist of GH activity both in vitro and in vivo. In the present study, human (h) GH analogs with alterations in the third alpha-helix (G120A, G120R) and N terminus (I4A) were generated. These two regions have been reported to form binding site 2 in hGH, which is involved in in vitro dimerization of the GH...