The roe deer is a monoestrous species with very short rutting season. present work reports the most suitable period for collecting epididymal sperm and describes effect of two cooling rates on post-thaw quality sperm. Testes were collected 24–48 h after death. Samples flushed from epididymis subjected to either (1) dilution in Tris-citric acid-glucose-egg yolk-based medium glycerol, slow freezi...

Eriksson, B. 2000. Cryopresewation of boar semen. Studies on sperm viability in vitro and fertility. Doctoral thesis. ISSN 1401-6257. ISBN 91-576-5903-6 The world’s pig population is consistently being upgraded through the international trade of superior genetics. The two major systems that are used for this purpose are the transport of live animals and the export of frozen boar semen. The main...

Conventional sperm freezing methods perform best when freezing sperm samples containing at least hundreds of spermatozoa. In this severe male factor infertility case series, we examined the reproductive outcomes in 12 intracytoplasmic sperm injection cases where spermatozoa used were frozen in Cell Sleepers. Cell Sleepers are novel devices in which individual spermatozoa can be frozen in microd...

REsPONSE oF SPERMATOZOA to freezing varies according to species. Hoagland and Pincus observed that rabbit spermatozoa could scarcely revive following immersion in liquid nitrogen after various plasmolytic pretreatment, whereas human spermatozoa remained highly active after freezing and thawing. It has been reported that rabbit spermatozoa are easily damaged by hypertonic salt solutions at tempe...

The study evaluated the protective effect of seminal plasma (SP) added to freezing extender against cryopreservation injuries to boar spermatozoa. Pooled sperm-rich fractions collected from 9 fertile boars were frozen in 0.5-mL straws after being extended in a conventional freezing extender either alone or supplemented with 5% of SPs (SP1-SP4) collected from the sperm-rich fractions (diluted 1:...

The present study was conducted to evaluate the in vivo fertility of frozen thawed boar semen. Twenty ejaculates collected from six mature boars were frozen in a programmable freezer. After freezing the semen was evaluated for different sperm quality parameters. Twenty five sows were inseminated artificially utilizing frozen thawed semen. The percentage of sperm motility, live sperm, live intac...

OBJECTIVE We investigated the activation of caspases 8, 9, 1, and 3 in human ejaculated spermatozoa to study main pathways of apoptosis. Potential functional impact of this phenomenon and possible activation mechanisms were examined by [1] subjecting cells to freezing and thawing, and [2] testing the dependence of caspase activity on membrane integrity. DESIGN Experimental study. SETTING An...

Cryopreservation is an efficient way to store spermatozoa and plays a critical role in the livestock industry as well as in clinical practice. During cryopreservation, cryo-stress causes substantial damage to spermatozoa. In present study, the effects of cryo-stress at various cryopreservation steps, such as dilution / cooling, adding cryoprtectant, and freezing were studied in spermatozoa coll...

introduction: the purpose of this research was to study the effects of two cryoprotectants on viability, motility, morphology and fertilization rate of mouse sperm after freezing-thawing. material and methods: spermatozoa were isolated from caudae epididymis of nmri male mice (8 to 10 weeks-old) and divided to three groups of control, experimental 1 and 2. cryoprotectant solution for the first ...

Both sperm and embryo cryopreservation have become routine procedures in human assisted reproduction and oocyte cryopreservation is being introduced into clinical practice and is getting more and more widely used. Embryo cryopreservation has decreased the number of fresh embryo transfers and maximized the effectiveness of the IVF cycle. The data shows that women who had transfers of fresh and f...