BACKGROUND/AIM Heat shock proteins (HSPs) are expressed in human spermatozoa and play a role in sperm function. MicroRNAs (miRNAs) regulate gene expression, and the possible involvement of miRNAs in the regulation of HSP gene expression in sperm was investigated in this study. MATERIALS AND METHODS miRNAs differentially expressed in 8 copies of an oligoasthenozoospermic semen group (OA) were ...

background: ‍‍cryopreservation has some detrimental impacts on sperms surface molecules. modification of the sperm surface molecules can affect on fertility rate. one of the important surface molecules are glycoconjugates. objective: the objective of this study was to evaluate the changes of content of the glycocalyx after standard cryopreservation procedure. materials and methods: forty five h...

the capability to preserve human oocytes, blastocysts, ovarian tissue and spermatozoa is an important tool in human assisted reproductive techniques. this preservation allows patients undergoing chemotherapy or radiotherapy to preserve their fertility, and helps to attain all benefits from the costly ovarian superovulation therapies prior to art. the primary goal in establishing an appropriate ...

Background: Current microscopic experimental methods cannot diagnose DNA damages present in spermatozoa .Therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. As reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. Also, chromomycin A3 is used for evaluation of the ...

We developed a new method to identify the separate cellular compartments in the optical path delay (OPD) maps of un-labeled spermatozoa. This was conducted by comparing OPD maps of fixed, un-labeled spermatozoa to bright field images of the same cells following labeling. The labeling enabled us to identify the acrosomal and nuclear compartments in the corresponding OPD maps of the cells. We the...

Progressively motile spermatozoa were present in 42/47 (89%) of post-vasectomy specimens, and in 16 of 24 samples (66%) demonstrable fertilizing potential could be detected in the zona-free hamster egg assay. The mean +/- s.e.m. post-vasectomy penetration rate of 20.6 +/- 6.0% was, however, significantly lower (P less than 0.001) than the pre-vasectomy value of 49.7 +/- 6.4%. After vasectomy th...

BACKGROUND Human spermatozoa appear to be guided by chemotaxis to the oocyte in the female genital tract. While one of the sources of sperm chemoattractants is the cumulus cells that surround the oocyte, the identity of the chemoattractant secreted from them is unknown. Progesterone, recognized to be secreted from cumulus cells, was demonstrated, at the pM concentration range, to be a chemoattr...

Crude decoction of Chenopodium album seed showed spermicidal effect at MIC 2 mg/ml in earlier studies. Systematic isolation, characterization and evaluation revealed that the major metabolite Desgalactotigonin (DGT) is the most effective principle in both in vitro and in vivo studies. The in vitro studies comprises (a) rat and human sperm motility and immobilizing activity by Sander-Cramer assa...