of Purpose: A comparison of long term effect on IOP and complications trabeculectomies performed with the adjunct of either postoperative 5 fluorouracil(5FU) or peroperative mitomycin C(MMC) in 100 cases of high risk glaucoma. &&&We prospectwly studied 52 eyes of46 patients treated with MMC (Follow up 15i8.7111) and compared it with a retrospective scrie of48 eyes 01 35 patients treated with 5F...

PURPOSE To investigate the expression of chemokines and their signaling pathways after application of mitomycin C (MMC) to corneal fibroblasts. METHODS Primary porcine and human corneal fibroblasts from passages 3 to 6 were treated with MMC at concentrations of 0.05, 0.1, or 0.2 mg/mL for 1, 2, 5, or 10 minutes. The relative expression of interleukin-8 (IL-8) and monocyte chemoattractant prot...

of Purpose: A comparison of long term effect on IOP and complications trabeculectomies performed with the adjunct of either postoperative 5 fluorouracil(5FU) or peroperative mitomycin C(MMC) in 100 cases of high risk glaucoma. &&&We prospectwly studied 52 eyes of46 patients treated with MMC (Follow up 15i8.7111) and compared it with a retrospective scrie of48 eyes 01 35 patients treated with 5F...

The bioreductive activation of mitomycin C (MMC) has been investigated using 10 human cancer cell lines. Except for 2 lines (COLO201 and COLO320DM), the cellular NAD(P)H: quinone oxidoreductase (DTD) activities correlated well with MMC-induced DNA damage and cytotoxicity. In addition, when the DTD activity was inhibited with 50 mM dicoumarol, the MMC activity decreased significantly. On the oth...

of Purpose: A comparison of long term effect on IOP and complications trabeculectomies performed with the adjunct of either postoperative 5 fluorouracil(5FU) or peroperative mitomycin C(MMC) in 100 cases of high risk glaucoma. &&&We prospectwly studied 52 eyes of46 patients treated with MMC (Follow up 15i8.7111) and compared it with a retrospective scrie of48 eyes 01 35 patients treated with 5F...

Glucose regulatory protein (GRP58) is known to mediate mitomycin C (MMC)-induced DNA cross-linking. However, the mechanism remains elusive. We hypothesized that thioredoxin-like domains, one at NH2 terminus and another at COOH terminus, are required for GRP58-mediated MMC reductive activation leading to DNA cross-linking. Site-directed mutagenesis mutated cysteines in thioredoxin domains to ser...

Glutathione (GSH) has been shown to modulate the Cytotoxicity of a variety of chemotherapeutic agents. The effect of mitomycin C (MMC) treatment duration and the effect of GSH depletion on in vitro cytotoxicity against the human colon cancer cell line 111-29 »asstudied under aerobic conditions. Continuous-exposure experiments revealed that the Cytotoxicity of 0.1 JIMMMC, as measured by clonoge...

We measured mitomycin C (MMC) concentrations in ocular tissues in rabbits with variable dose (0.1, 0.2, or 0.4 mg) and duration of application time (1, 3, or 5 minutes) of MMC using high-performance liquid chromatography. Mitomycin C concentrations at the administered site after single subconjunctival application of MMC and after irrigation showed significant correlation with dose and duration ...

of Purpose: A comparison of long term effect on IOP and complications trabeculectomies performed with the adjunct of either postoperative 5 fluorouracil(5FU) or peroperative mitomycin C(MMC) in 100 cases of high risk glaucoma. &&&We prospectwly studied 52 eyes of46 patients treated with MMC (Follow up 15i8.7111) and compared it with a retrospective scrie of48 eyes 01 35 patients treated with 5F...

PURPOSE To study the aqueous and corneal pharmacokinetics of mitomycin C (MMC) after single topical administration to the central cornea and to evaluate the effects of different concentrations and different application times on the aqueous concentration of MMC. METHODS Mechanical epithelium debridement of the central 7.5 mm of the cornea was performed in New Zealand white rabbits, and a spong...