Products containing probiotic bacteria are gaining popularity, increasing the importance of their accurate speciation. Unfortunately, studies have suggested that improper labeling of probiotic species is common in commercial products. Species identification of a bank of commercial probiotic strains was attempted using partial 16S rDNA sequencing, carbohydrate fermentation analysis, and cellular...

*Correspondence: J. N. Chung, Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL 32611, USA e-mail: [email protected] Two concept systems that are based on the thermochemical process of high temperature steam gasification of lignocellulosic biomass and municipal solid waste are introduced.The primary objectives of the concept systems are (1) to develop the ...

Superadiabatic Partial Oxidation for Hydrogen and Sulfur Production from Hydrogen Sulfide The Gas Technology Institute has been investigating superadiabatic partial oxidation concepts to not only develop novel, cost-effective processes to recover hydrogen and sulfur from hydrogen sulfide, but also produce hydrogen from natural gas and other liquid hydrocarbons, and gasify solid feedstocks, incl...

The present invention provides a cost-effective method of producing pure hydrogen gas from hydride-based solid materials. The hydride-based solid material is mechanically processed in the presence of a catalyst to obtain pure gaseous hydrogen. Unlike previous methods, hydrogen may be obtained from the solid material without heating, and without the addition of a solvent during processing. The d...

BACKGROUND While microbial electrolysis cells (MECs) can simultaneously produce bioelectrochemical hydrogen and treat wastewater, they consume considerable energy to overcome the unfavorable thermodynamics, which is not sustainable and economically feasible in practical applications. This study presents a proof-of-concept system in which hydrogen can be produced in an MEC powered by theoretical...

Enhanced Hydrogen Production in Escherichia coli Through Chemical Mutagenesis, Gene Deletion, and Transposon Mutagenesis. (May 2010) Andrea Juliana Garzon Sanabria, B.S., Universidad Industrial de Santander at Bucaramanga Chair of Advisory Committee: Dr. Thomas K. Wood We demonstrate that hydrogen production can be increased by random mutagenesis using N-methyl-N ́-nitro-N-nitrosoguanidine (MNNG...

BACKGROUND The future hydrogen economy offers a compelling energy vision, but there are four main obstacles: hydrogen production, storage, and distribution, as well as fuel cells. Hydrogen production from inexpensive abundant renewable biomass can produce cheaper hydrogen, decrease reliance on fossil fuels, and achieve zero net greenhouse gas emissions, but current chemical and biological means...

Hydrogen fuel is renewable, efficient and clean, and fermentative bacteria hold great promise for its generation. Here we use the isogenic Escherichia coli K-12 KEIO library to rapidly construct multiple, precise deletions in the E. coli genome to direct the metabolic flux towards hydrogen production. Escherichia coli has three active hydrogenases, and the genes involved in the regulation of th...