A tumor-associated transplantation antigen with an apparent molecular weight of 75,000 has been isolated from the cytosol of the BALB/c methylcholanthrene-induced sarcoma, Meth A. The antigen was purified either by preparative electrophoresis in the presence of NaDodSO4 or by immunoaffinity chromatography after hexylamine agarose chromatography, gel filtration, and hydroxylapatite chromatograph...

Laser ablation of solids in liquids technique has been used to obtain colloidal nanoparticles from biological hydroxylapatite using pulsed as well as a continuous wave (CW) laser. Transmission electron microscopy (TEM) measurements revealed the formation of spherical particles with size distribution ranging from few nanometers to hundred nanometers and irregular submicronic particles. High reso...

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a 38-amino acid peptide (PACAP38) or a truncated peptide with the same 27 amino-terminal residues (PACAP27). The PACAP receptor was solubilized from bovine brain membranes with digitonin and purified 30-fold by the combination of DEAE-Toyopearl and hydroxylapatite chromatographic analyses. The partially purified PACAP receptors were ...

IMPORTANCE Even when administered by experienced hands, injectable soft-tissue fillers can cause various unintended reactions, ranging from minor and self-limited responses to severe complications requiring prompt treatment and close follow-up. OBJECTIVES To review the complications associated with injectable soft-tissue filler treatments administered in the Williams Rejuva Center during a 5-...

Pesticin was prepared from cell-free extracts of Yersinia pestis strain All22 by a procedure involving fractionation with (NHa)nS04 and chromatography with Sephadex G-200, diethylaminoethylcelulose, and calcium hydroxylapatite. A single antibacterial fraction (a-pesticin) was recovered from extracts of uninduced yersiniae, whereas extracts of mitomycin C-induced cells yielded an additional acti...

Radiesse (Bioform Inc, USA) is a sterile, latex-free, non-pyrogenic, semi-solid, cohesive subdermal, injectable implant, whose principal component is synthetic calcium hydroxylapatite, a biocompatible material with over 20 years of use in medicine. The semi-solid nature of the product is created by suspending calcium hydroxylapatite microspheres of 25-45 microns diameter in a gel carrier of car...

C-proteins have been isolated from rabbit red skeletal muscle (soleus and semitendinosus) and cardiac muscle and their structure and properties compared with those of white muscle C-protein. The Mr of white, red, and cardiac C-proteins are 135,000, 145,000, and 150,000, respectively, and their s20,w values are 4.3, 3.8, and 4.8 S, indicating that red C-protein is more asymmetric than the other ...

28 Metal immobilization may contribute to the environmental management strategy of 29 dredged sediment landfill sites contaminated by metals. In a field experiment, 30 amendment effects and efficiency were investigated, focusing on plants, springtails and 31 bacteria colonisation, metal extractability and sediment ecotoxicity. Conversely to 32 hydroxylapatite (HA, 3 % DW), the addition of Thoma...

The general procedure for the isolation of the phosphate carrier from mitochondria involves solubilization with non-ionic detergents and chromatography on hydroxylapatite [l-3]. With high resolution SDS-gel electrophoresis this preparation can be separated into 4-5 protein bands [2]. Further purification, in particular removal of the ADP/ATP-carrier, was obtained by chromatography on Celite [2]...