Although immunoassays are the de facto standard for determining subcellular protein localization in individual cells, antibody probe cross-reactivity and fixation artifacts remain confounding factors. To enhance selectivity while providing single-cell resolution, we introduce a subcellular western blotting technique capable of separately assaying proteins in the 14 pL cytoplasm and 2 pL nucleus...

Targeting the activation of immune checkpoints is recognized as an effective strategy for triggering anti-tumour responses in cancer cells. Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and programmed cell death 1 (PD-1) were identified potential crucial targets treatment. Overexpression CTLA-4 PD-1 proteins primary tumour human lines well documented. In contrary, lack data was available...

Hydatidosis is a disease caused by infection with the metacestode stage of the dog tapeworm,Echinococcus granulosus. This is recognised as one of the world’s most important zoonoses, affecting bothhumans and their domestic animals. In the current study, 20 sheep cystic livers or lungs were collected.Hydatid fluid and protoscolices were isolated and the soluble protein was prepared. Polypeptide ...

von Willebrand factor (vWF) was purified from pooled normal plasma, radiolabeled with iodine then cleaved by porcine pancreatic elastase. Cleavage was monitored by sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE), SDS-agarose electrophoresis, crossed immuno-electrophoresis, ristocetin and botrocetin cofactor activities, and ristocetin and botrocetin induced binding to fixed ...

INTRODUCTION We present our work of monitoring 202 different patients with markedly elevated serum index for lipemia whereby serum samples were clear. We tried to clarify the cause of occurrence of these indices which were detected in the years 2006-2010 on Siemens Dimension analyzers. MATERIALS AND METHODS In samples with unusual lipemia index we measured the concentration of lipids (total c...

it is well accepted that in experimental model of leishmania major infection, balb/c mice mount a th2 response and produce igg1 predominantly whereas c57bl/6mice enhance th1 response with the biased production of igg2a antibodies. therefore, screening for parasite antigens on the basis of reactivity with sera from infected susceptible or resistant mice might be used for the identification of th...

Troponin-T (Tn-T) expression in developing hearts of axolotls, Ambystoma mexicanum, was studied with the use of polyclonal and monoclonal antibodies and SDS-polyacrylamide gel electrophoresis. In precontractile hearts (stage 32/33), Tn-T was present in addition to myosin, actin and tropomyosin as evidenced by the presence of the protein bands in SDS-gels and by indirect immunofluorescence. Tn-T...