An enzyme-linked immunosorbent assay was developed to detect antibodies to infectious bovine rhinotracheitis virus in bovine sera. The test used viral antigen purified in a sucrose density gradient, and the antibody titers detected were up to 100 times higher than those seen in the serum neutralization test. When 250 sera were tested by those two tests, 146 were positive and 49 were negative in...

In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system. The positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (D...

We evaluated a commercially available enzyme immunoassay method for determining the concentration of total diazepam plus its active metabolite, nordiazepam, in small amounts of serum and urine. The coefficients of variation were 5% within assay and 8% between assay. Concentrations so measured for patients' sera correlated well with those obtained both by gas chromatography with electron-capture...

Vol. 26, No. 12, 1980 3. Jaklitsch, A. P., Schneider, R. S., Johannes, R. J., et aL, Homogeneous enzyme immunoassay for T-4 in serum. C/in. Chem. 22,1185 (1976). Abstract. 4. Galen, R. S., and Forman, D., Enzyme immunoassay of serum thyroxine with the “Auto Chemist” multichannel analyzer. C/in. Chem. 23, 119-121 (1977). 5. Finley, P. R., and Williams, R. J., Evaluation of homogeneous enzyme imm...

We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Ou...

In this work, a fluorescence signal transduction mechanism based on cation release from ZnS nanocrystals was developed for sandwich immunoassay. In this mechanism, ZnS nanocrystals as labels in immunoassay are dissolved by acid to release zinc ions. After pH adjustment of the dissolving solution using a basic solution, zinc-ion sensitive fluorescence indicator Fluozin-3 is added to bind with th...