We have studied the immunochemical and biochemical differences in 12 Aspergillus fumigatus strains isolated from different sources. The enzymatic activity of all these strains were studied by a rapid enzyme detection method (API-ZYM). One of 12 strains studied produced alkaline phosphatase, while two produced chymotrypsin, and three produced trypsin. By SDS-PAGE we studied proteins present in t...

When it is incompletely saturated with iron, transferrin may exist in four molecular forms: apotransferrin, monoferric (A) transferrin (with iron occupying only the A site of the protein), monoferric (B) transferrin, and diferric transferrin. By combining electrophoresis in urea-polyacrylamide gels with crossed immunoelectrophoresis using specific antihuman transferin antiserum, it is possible ...

Two-dimensional immunoelectrophoresis (2D-IEP), in which a complex of antigens is subjected to electrophoresis first through an agarose matrix in one direction and secondly through an antiserum-agarose matrix at right angles to the first direction, was evaluated as a tool for analysis of mycobacterial antigens. Cell extracts from four species of mycobacteria, Mycobacterium tuberculosis (four st...