The aim of this study is to elaborate a simple and sensitive electrochemical immunoassay using ferrocenecarboxylic (Fc-COOH)-doped silica nanoparticles (SNPs) as an immobilized affinity support for cancer antigen 15-3 (CA 15-3) detection. The Fc-COOH-doped SNPs with redox-active were prepared by using a water-in-oil microemulsion method. The use of colloidal silica could prevent the leakage of ...

Celiac disease is a gluten-induced autoimmune enteropathy characterized by the presence of tissue tranglutaminase (tTG) autoantibodies. A disposable electrochemical immunosensor (EI) for the detection of IgA and IgG type anti-tTG autoantibodies in real patient's samples is presented. Screen-printed carbon electrodes (SPCE) nanostructurized with carbon nanotubes and gold nanoparticles were used ...

One novel electrochemical immunosensor was constructed by immobilizing capture antibody of alpha-fetoprotein (AFP Ab(1)) on a nafion/nanogold-particle modified glassy carbon electrode. With a sandwich immunoassay, one DNA-derived magnetic nanoprobe, simplified as DNA/(ZMPs-HRP-AFP Ab(2))(n), was employed for the detection of AFP. The fabricated procedure of the proposed biosensor was characteri...

Reduced graphene oxide (RGO) has recently gained considerable attention for use in electrochemical biosensing applications due to its outstanding conducting properties and large surface area. This report presents a novel microfluidic chip integrated with an RGO-based electrochemical immunosensor for label-free detection of an influenza virus, H1N1. Three microelectrodes were fabricated on a gla...

An immunosensor is a device comprising an antigen or antibody species coupled to a signal transducer, which detects the binding of the complementary species. An indirect immunosensor uses a separate labeled species that is detected after binding by, e.g., fluorescence or luminescence (i.e., a heterogeneous immunoassay). A direct device detects the binding by a change in potential difference, cu...

A novel strategy to quantify the cell number of leukemia K562A cells using electrochemical immunosensor was developed by effective surface immunoreaction between P-glycoprotein (P-gp) on cell membrane and P-gp mouse monoclonal antibody bound on an epoxysilane monolayer modified glassy carbon electrode. The surface morphologies of the epoxysilane monolayer and the bound antibodies were studied w...

Polyhydroquinone-graphene composite as a new redox species was synthesized simply by a microwave-assisted one-pot method through oxidative polymerization of hydroquinone by graphene oxide, which exhibited excellent electrochemical redox activity at 0.124 V and can remarkably promote electron transfer. The as-prepared composite was used as immunosensing substrate in a label-free electrochemical ...

Immunoglobulin Y is a water-soluble protein present in high concentration in hen serum and egg yolk. IgY has applications in many fields, e.g., from food stuff to the mass production of antibodies. In this work, we have implemented an electrochemical immunosensor for IgY based on templated nanoelectrodes ensembles. IgY is captured by the templating polycarbonate and reacted with anti-IgY labele...

A waveguide Mach-Zehnder Interferometer (MZI) immunosensor has been developed which can detect, in a direct way, a minimum average layer growth thickness of the analyte of 2X 10e3 nm (bound mass, I’=lX low4 fig cm-“). The design and fabrication of the sensor and the experimental set-up are aimed at decreasing the detection limit. A flow cell has been designed and incorporated into the set-up to...

An enzymatic reaction was employed as a means to enhance the sensitivity of an immunosensor based on localized surface plasmon resonance (LSPR). The reaction occurs after intermolecular binding between an antigen and an antibody on gold nano-island (NI) surfaces. For LSPR sensing, the gold NI surface was fabricated on glass substrates using vacuum evaporation and heat treatment. The interferon-...