We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.

Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

Standard interpretations of Kant’s transcendental idealism take it as a 1 commitment to the view that the objects of cognition are structured or made by con2 ditions imposed by the mind, and therefore to what Van Cleve calls “honest-to-God 3 idealism”. Against this view, many more recent investigations of Kant’s theory of rep4 resentation and cognitive significance have been able to show that K...

“Wild thorns” is a novel written by the Palestinian novelist Sahar Khalifeh; it focuses on the colonisation of Palestine from a feminist point of view. She considers the Israeli colonisation as a patriarchy. This paper aims at investigating two types of colonization that have been represented in the form of patriarchy in the novel. The investigation of the issue of Palestine from a feminist poi...

since ethics is one of the subdivisions of practical wisdom, islamic philosopher’s view point is very important in explanation of theoretical and practical reason. this paper is going to analyze the distinction between practical and theoretical reason from haeri’s point of view. in order to finding the roots of his issue, at the beginning we will propose the idea of philosophers like farabi, ib...

death is something real which is fully accepted by human being, the definition of death is different among public and scientists such as philosophy experts and biologists. here we leave the philosophy content to themselves and look at the death from the biological point of view which may be some kind of philosophy as well. in biological science the death is well defined so it is the end of life...

The function of pslD, which is part of the psl operon from Pseudomonas aeruginosa, was investigated in this study. The psl operon is involved in exopolysaccharide biosynthesis and biofilm formation. An isogenic marker-free pslD deletion mutant of P. aeruginosa PAO1 which was deficient in the formation of differentiated biofilms was generated. Expression of only the pslD gene coding region resto...