In vitro microbial contaminations are the most serious problems in plant tissue culture. Initial attempts for micropropagation of Dionysia tapetodes, a beautiful alpine cushion plant from north-east Iran, led to 100% explants contamination. Hence, the present study was conducted to develop an efficient surface sterilization protocol for in vitro culture of field collected D. tapetodes using com...

background: vitrification has proven to be more effective than slow freezing methods to cryopreserve mammalian oocytes. the objectives of this study were to evaluate the effects of vitrification on immature and in vitro matured, denuded and cumulus compact goat oocytes and their subsequent fertilization. methods: oocytes were either cryopreserved as immature cumulus compact (imcc) (n=98 exp 1; ...

background: it is important to protect oocytes and embryos from oxidative stress in the culture medium. melatonin has been shown to be a direct free radical scavenger. objective: effect of melatonin during in vitro oocyte maturation, fertilization and embryo development of mouse oocytes was evaluated. materials and methods: oocytes from supper-ovulated mouse were divided to two groups: cumulus ...

Objective: In vitro propagation of AZ x M9 new apple rootstock resulted of breeding program of vegetative apple rootstock in Iran was investigated. Methods: Nodal explants were inoculated in Murashige and SKoog (MS) medium supplemented with various concentrations of 6-benzylaminon purine (BAP) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (NAA) (0.1 mg/l). We examined t...

Background & Aim: Persian shallot (Allium hirtifolium Boiss) with common name of "Musir" is one of the endemic medicinal plants in Iran. Leaves and bulbs with antibacterial, antifungal, anticancer, anti-diabetes and anti-obesity effects are edible parts of this plant. The tissue culture is an influential method to multiply plant productions in order to meet demands of ...

Two separate factorial experiments were conducted to study the effects of explant sources, plant growth regulators, sucrose concentrations, and light conditions on in vitro cormlet formation of freesia (Freesia ×hybrida Bailey 'Argenta'). Interestingly, it was observed that the pupae had lower contamination levels compared to mother corms. Using 40% sodium hypochlorite solution for 40 min, cont...

objective: in vitro ovarian follicle culture provides a tool to investigate folliculogenesis and may be used as an assisted reproductive technology )art(. this study aims to compare survival and development rates in mouse ovarian follicles after two and three dimensional in vitro cultures. methods: preantral follicles were isolated from the ovaries of 14-day old female mice and cultured in α-m...