Bilirubin uptake by the liver is a rapid process of high specificity that has kinetic characteristics which suggest carrier-mediation. In the circulation, bilirubin is readily bound to albumin, from which it is extracted by the liver. Although several studies suggested that it is the small, unbound fraction of bilirubin which interacts with hepatocytes and is removed from the circulation, recen...

Sucrose uptake was studied in isolated, immature pea cotyledons (Pisum sativum L. cv Marzia) in relation to their developmental stage. During the developmental period examined the water content of the cotyledons decreased from approximately 80% "stage 1" to approximately 55% "stage 2". When assayed in an isotonic medium (400 osmoles per cubic meter) the influx capacity per gram fresh weight for...

1'-Fluorosucrose (FS), a sucrose analog resistant to hydrolysis by invertase, was transported from husk leaves into maize (Zea mays L., Pioneer Hybrid 3320) kernels with the same magnitude and kinetics as sucrose. (14)C-Label from [(14)C]FS and [(14)C]sucrose in separate experiments was distributed similarly between the pedicel, endosperm, and embryo with time. FS passed through maternal tissue...

an in vitro experiment was conducted to determine the effect of supplemental non-fiber carbohydrate(nfc) on the disappearance kinetics of cellulose (ce) by mixed ruminal microorganisms. non-supplementedor nfc supplemented cellulose (467 mg nfc/g cellulose as sucrose (cesu) or starch (cest) or a 1:1 mixtureof sucrose + starch (cesust)) were incubated for 24, 48, and 96 h at 39°c. after each incu...

The aim of this study was to comparatively analyze chemical composition and biological activity wild- in vitro grown Rindera umbellata. Explants were cultivated on 0.003–0.3 M sucrose, fructose, or glucose. HPLC-DAD for quantifying rosmarinic (RA) lithospermic B (LAB) acids GC-MS/FID qualitative pyrrolizidine alkaloids (PAs) detection used. Antioxidant (DPPH ABTS assays) cytotoxicity (MTT test)...

Enzymatically isolated vein networks from mature pea (Pisum sativum L. cv Alaska) leaves were employed to investigate the properties of sucrose loading and the effect of phytohormones and cell turgor on this process. The sucrose uptake showed two components: a saturable and a first-order kinetics system. The high affinity system (K(m), 3.3 millimolar) was located at the plasmalemma (p-chloromer...

Sucrose is the main saccharide used for long-distance transport in plants and plays an essential role energy metabolism; however, there are no analogues real-time imaging live cells. We have optimised a synthetic approach to prepare sucrose including very small (?50 Da or less) Raman tags fructose moiety. Spectroscopic analysis identified alkyne-tagged compound 6 as analogue recognised by endog...

The present investigation was laid out in Completely Randomized design (CRD) with 10 treatments and each replicated thrice. treatment T7 ‘Sucrose 2% + Aluminium sulphate @ 200 ppm reported significantly better performance compared to other treatments, terms of water uptake (6.79g/stem), flower diameter (6.00 cm), weight spikes (42.50 g), spike length (11.50 (2.33 mm), total solution consumption...

Two Citrus sinensis (L.) phosphomannose isomerase (PMI) genes, CsPMI1 and CsPMI2, were evaluated as novel selectable markers in mature citrus transformation. Transgenic shoots produced after transformation of Kuharske rootstock with each PMI construct selected on six treatments mannose sucrose. For CsPMI1, there no significant differences among the various sucrose for mean number positive (PS),...