As there was not any previous comprehensive study on the serological tests for hepatic amebiasis in Iran, the indirect hemagglutination (IHA), indirect fluorescent antibody (IF A), and biocolored latex agglutination (BLA) tests were evaluated in the serodiagnosis of amebic liver abscess(ALA). For this purpose a total of 165 serum samples 18 of which were obtained from patients known to have...

Fluorescent antibody methods were originally introduced by Coons and associates in 1942. Included in this first publication was a procedure for antibody-fluorescein conjugation and a description of fluorescent reactions obtaincd by means of ultraviolet light microscopy. This communication, and later ones by other authors, indicated that fluorescent antibody procedures were practical for rapid a...

19 The antemortem diagnosis of rabies in humans employs techniques that require 20 accuracy, speed and sensitivity. A combination of histochemical analysis, in vitro virus isolation, 21 immunologic methods and molecular amplification procedures are utilized in an effort to 22 diagnose the disease. Modern medicine now offers potentially life-saving treatment for a disease 23 that was considered ...

BACKGROUND The IFA test is one of the most usual methods for detecting anti-Toxoplasma antibodies, although it has not any unique standardization. It seems that the microscopic judgment of results is an important confounder in IFA test. Therefore, we conducted the present study to clarify the role of microscopic observer, and other confounders on the test. METHODS Eighty sera were collected f...

Seventy percent of pneumococci isolated from the middle-ear cavity of infants and children with acute otitis media were of one of the seven serotypes 1, 3, 6, 14, 18, 19, or 23. The immunological response in the serum and middle-ear fluid from otitis media caused by one of these serotypes was studied in 61 children by using either indirect hemagglutination or indirect fluorescent antibody tests...

Chroust K., Mittermayer T.: Indirect Fluorescent Antibody Test in Diagnosis of Trichinellosis in Man. Acta vet. Brno, 52, 1983: 177 -181. To demonstrate the presence of antibodies during the six trichinellosis epidemics in humans in the east Slovakia in 1971-1980 the indirect fluorescent antibody test (IFA), the microprecipitation reaction (MP) and the complement fixation reaction (CF) were emp...

Sm14 is a 14-kDa vaccine candidate antigen from Schistosoma mansoni that seems to be involved in cytoplasmic trafficking of fatty acids. Although schistosomes have a high requirement for lipids, they are not able to synthesize fatty acids and sterols de novo. Thus, they must acquire host lipids. In order to determine whether Sm14 is present in different stages of the life cycle of the parasite,...

OBJECTIVE To study the presence and levels of GAD65 antibodies (GADA), IA-2 antibodies (IA-2-A), and islet cell antibodies (ICA) during the first years after clinical onset of type 1 diabetes in relation to age at diagnosis. RESEARCH DESIGN AND METHODS Type 1 diabetic patients (n = 194) <40 years of age were consecutively recruited at the time of diagnosis by the Belgian Diabetes Registry and...

AIMS To investigate the serological cross reaction between legionella and campylobacter using the rapid microagglutination test (RMAT). METHODS Serum samples from 49 patients with campylobacter infection were tested for legionella antibodies using the indirect fluorescent antibody test (IFAT) and the RMAT. Serum samples that had positive RMAT titres were retested in the presence of a campylob...