the aim of this study was to investigate the in vitro developmental competence of isolated pre-antral follicles derived from vitrified ovaries in the presence of coenzyme q 10 (coq 10 ) . mice pre-antral follicles derived from fresh and vitrified-warmed ovarian tissues were in vitro cultured individually in α-mem medium supplemented with or without coq 10 , followed by adding human chorionic go...

Ebola virus (EBOV) infections are characterized by deficient T lymphocyte responses, T lymphocyte apoptosis, and lymphopenia in the absence of direct infection of T lymphocytes. In contrast, dendritic cells (DC) are infected but fail to mature appropriately, thereby impairing the T cell response. We investigated the contributions of EBOV proteins in modulating DC maturation by generating recomb...

AIM To assess proliferative and apoptotic potential of the seminiferous epithelium cells in relation to Sertoli cell maturation in newborn rats under the influence of estradiol, follicle stimulating hormone (FSH) or both agents given together. METHODS From postnatal day (PND) 5 to 15 male rats were daily injected with 12.5 microg of 17beta-estradiol benzoate (EB) or 7.5 IU of human purified F...

The substance procarbazine (Natulan®) is a teratogen known to induce cleft palates in rats on day 14 post-conception. The application of thiocyanate (SCN-) alone to rats on day 10 and 14 of pregnancy had no effects on fetal bone maturation. However, when procarbazine was used, the maturation and growth of fetal bones was delayed. Upon additional application of thiocyanate, the effects of procar...

Cytoplasmic extracts of meiotically mature mouse oocytes were injected into immature Xenopus laevis oocytes, which underwent germinal vesicle breakdown within 2 h. Germinal vesicle breakdown was not inhibited by incubation of the Xenopus oocytes in cycloheximide (20 micrograms/ml). Identically prepared extracts of meiotically immature mouse oocytes, arrested at the germinal vesicle stage by dib...

UNLABELLED BioBran, enzymatically modified arabinoxylan from rice bran was tested for its possible effects on in vitro maturation of human dendritic cells (DC). Immature DC (iDC) derived from plastic-adhered, IL-4 and GM-CSF treated peripheral monocytes (Mo) were further cultured with cytokine maturation mix 1 (CMM1; TNF-alpha, IL-1beta and IL-6) or CMM2 (LPS and IFN-gamma) to induce their matu...

In amphibians, as well as in other vertebrates, occytes start to mature under the action of hypophyseal gonadotropic hormones. (In this paper the term 'maturation' implies the transformation into ripe eggs of oocytes that have finished growing.) In the course of maturation the oocytes themselves and the follicle cells surrounding them undergo changes; the changes of these two cell types are not...

the aim of the study was to compare the parthenogenetic activation and in vitro fertilization (ivf) of in vitro matured caprine oocytes. a total of 881 cumulus-oocyte complexes (coc’s) were collected from 243 ovaries. oocytes were matured in tcm-199 medium containing ecg (20 iu/ml), hcg (20 iuμg/ml), oestradiol-17β (1 μg/ml), bsa embryo tested (3 mg/ml) supplemented with 10% fetal bovine serum ...

Introduction. Maintenance of the articular surface depends on the function of articular chondrocytes (ACs) which produce matrix and are constrained from undergoing terminal maturation. Under some circumstances, such as in osteoarthritis, these constraints are lost and ACs enter the maturational program that occurs during endochondral ossification. The aim of this study was to identify these unk...