This study was aimed at producing protease and lipase simultaneously on a common medium by Bacillus licheniformis VSG1, which was isolated from a tannery effluent. The effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was analysed. Both those enzymes were produced under optimized conditions like pH, temperature and i...

Protein redesign methods aim to improve a desired property by carefully selecting mutations in relevant regions guided by protein structure. However, often protein structural requirements underlying biological characteristics are not well understood. Here, we introduce a methodology that learns relevant mutations from a set of proteins that have the desired property and demonstrate it by succes...

Microbial enzymes such as xylanases enable new technologies for industrial processes. Xylanases (xylanolytic enzyme) hydrolyze complex polysaccharides like xylan. Research during the past few decades has been dedicated to enhanced production, purification, and characterization of microbial xylanase. But for commercial applications detailed knowledge of regulatory mechanisms governing enzyme pro...

The several industrial applications of lipases have stimulated interest in isolation of new enzymes from novel sources. In this study, the lipase production from Trichoderma atroviride 676 was optimized by statistical design methods. The response surface methodology based on 3(3-1) fractional factorial, showed that the yeast extract and MgSO4 played a significant role on lipase production, yiel...

Search for new industrial enzymes having novel properties continues to be a desirable pursuit in enzyme research. The halophilic organisms inhabiting under saline/ hypersaline conditions are considered as promising source of useful enzymes. Their enzymes are structurally adapted to perform efficient catalysis under saline environment wherein n0n-halophilic enzymes often lose their structure and...

Microbial cellulases find applications in various industries and constitute a major group of the industrial enzymes. Recently, there is resurgence in utilization of biomass for fuel production employing cellulases and hence forth in obtaining better yields and novel activities. Improving the economics of such processes will involve cost reduction in cellulase production which may be achieved by...

background and objectives: lipases are valuable biocatalysts which are widely used in the detergent, food, dairy and pharmaceutical industries. the aims of the present study included the isolation of a lipase-producer from industrial zones and the partial characterization of the enzyme. materials and methods: a number of bacteria were isolated from sites related to the oil industries. an isolat...

in recent years, several oconjugation protocols have been developed to improve the functional properties of the enzyme lysozyme. in this study, the optimum conditions of lysozyme – oxidized inulin conjugation and functional properties of the modified lysozyme were investigated. initially, inulin (mw about 25 kda) was oxidized by periodate to provide the reactive carbonyl groups  to attach to am...