The effect of a rise in intracellular Ca(2+) concentration was analyzed in isolated rat olfactory neurons using a whole-cell patch-clamp technique. Intracellular dialysis of 1 mmol l(-)(1) Ca(2+) in a standard-K(+), low-Cl(-) internal solution (E(Cl)=-69 mV) from the patch pipette into the olfactory neurons induced a sustained outward current of 49+/-5 pA (N=13) at -50 mV in all the cells exami...

Larval molting in Drosophila, as in other insects, is initiated by the coordinated release of the steroid hormone ecdysone, in response to neural signals, at precise stages during development. In this study we have analyzed, using genetic and molecular methods, the roles played by two major signaling pathways in the regulation of larval molting in Drosophila. Previous studies have shown that mu...

The presence and physiological role of Ca 2 1 induced Ca 2 1 release (CICR) in nonmuscle excitable cells has been investigated only indirectly through measurements of cytosolic [Ca 2 1 ] ([Ca 2 1 ] c ). Using targeted aequorin, we have directly monitored [Ca 2 1 ] changes inside the ER ([Ca 2 1 ] ER ) in bovine adrenal chromaffin cells. Ca 2 1 entry induced by cell depolarization triggered a tr...

In many cell types agonist-receptor activation leads to a rapid and transient release of Ca(2+) from intracellular stores via activation of inositol 1,4,5 trisphosphate (InsP(3)) receptors (InsP(3)Rs). Stimulated cells activate store- or receptor-operated calcium channels localized in the plasma membrane, allowing entry of extracellular calcium into the cytoplasm, and thus replenishment of intr...

BACKGROUND This study was designed to assess endotracheal climate in laryngectomized patients in a warm and dry environment and the effects of a heat and moisture exchanger (HME). METHODS Endotracheal temperature and humidity were measured in 11 laryngectomized patients with a regularly used HME (Provox Normal HME; R-HME), an HME with antimicrobial filter (Provox Micron HME; F-HME), and witho...

In recent years, by extensive achievements in understanding the mechanisms and the pathways affected by cancer, the focus of cancer research is shifting from developing new chemotherapy methods to using natural compounds with therapeutic properties to reduce the adverse effects of synthetic drugs on human health. We used fruit extracts from previously generated human type I InsP 5-ptase gene ex...

This study aimed to evaluate the effects of soluble and insoluble non-starch polysaccharides (NSPs) on growth performance, digestive enzyme activity, antioxidant capacity, intestinal flora juvenile GIFT tilapia ( Oreochromis niloticus ). Four isonitrogenous isolipidic experimental diets were formulated, including FM diet (0% NSPs, FM), INSP (5.8% INSP), SNSP (12.2% SNSP), NSP 12.2% SNSP, NSPs)....

The murine BALB/c 3T3 fibroblast clone SV-T2 (3T3 cells) expresses receptors for the nonapeptide bradykinin. In these cells, bradykinin stimulates both inositol phosphate (InsP) formation and arachidonic acid release by independently activating phospholipase C and phospholipase A2, respectively. These actions of bradykinin are mediated by a receptor(s) coupled to pertussis toxin-insensitive gua...

Diverse patterns of Ca(2+)(i) release differentially regulate Ca(2+)-sensitive enzymes and gene transcription, and generally the extent of agonist activation of phospholipase C-linked G protein-coupled receptors determines the type of Ca(2+) signal. We have studied global Ca(2+) oscillations arising through activation of the metabotropic glutamate receptor mGluR5a expressed in Chinese hamster o...

220-kDa ankyrin-B is required for coordinated assembly of Na/Ca exchanger, Na/K ATPase, and inositol trisphosphate (InsP(3)) receptor at transverse-tubule/sarcoplasmic reticulum sites in cardiomyocytes. A loss-of-function mutation of ankyrin-B identified in an extended kindred causes a dominantly inherited cardiac arrhythmia, initially described as type 4 long QT syndrome. Here we report the id...