the latest micromanipulative technique, intracytoplasmic sperm injection (icsi), has been very successful in cases of severe male factor infertility. the main objective of this study is to report the results of our first experience with icsi in cases of male factor infertility, as well as patients who had failed their previous cycles with conventional in vitro fertilization (ivf). normal fertil...

Microsurgical epididymal sperm aspiration (MESA) refers to retrieval of sperm-containing fluid from optimal areas of the epididymis that are selected and sampled using high-power optical magnification provided by an operating microscope. Retrieved sperm are subsequently used for intracytoplasmic sperm injection (ICSI) to induce fertilization and pregnancy. MESA is considered by many experts to ...

The latest micromanipulative technique, intracytoplasmic sperm injection (ICSI), has been very successful in cases of severe male factor infertility. The main objective of this study is to report the results of our fIrst experience with ICSI in cases of male factor infertility, as well as patients who had failed their previous cycles with conventional in vitro fertilization (IVF). Normal ...

BACKGROUND ICSI into the oocyte is the only treatment currently available for most male patients with severe oligozoospermia who wish to father children. In order to perform ICSI, motile sperm need to be recovered from the ejaculate and, if no sperm or not enough motile sperm are recovered on the day of ICSI, testicular sperm extraction (TESE) must be performed. Oxytocin stimulates epididymal c...

BACKGROUND Sperm of poor quality can negatively affect embryo development to the blastocyst stage. The aim of this comparative prospective randomized study was to evaluate the role of an intracytoplasmic morphologically selected sperm injection (IMSI) in the same infertile couples included in the programme of intracytoplasmic sperm injection (ICSI) due to their indications of male infertility w...

background: sperm dna fragmentation is known as an important cause of male infertility. the influence of sperm dna damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial. in this study, we investigated differences of the semen parameters and intracytoplasmic sperm injection (icsi) outcome according to sperm dna fragme...

BACKGROUND There are still concerns about the safety of intracytoplasmic sperm injection (ICSI) due to its brief clinical record and lack of animal testing. Testicular and epididymal sperm are now used routinely for ICSI in patients with obstructive azoospermia. The use of such immature sperm compounds fears, since little is known of their mitochondrial and nuclear DNA quality. METHODS A modi...

INTRODUCTION In this study, we aimed to evaluate the safety of using different sources of sperm when male partners were infected with hepatitis B virus (HBV). MATERIAL AND METHODS A total of 338 couples receiving their first intracytoplasmic sperm injection (ICSI) cycle at the Department of Reproduction, Ren Ji Hospital, between 2007 and 2012 were enrolled if the female partner tested negativ...

In this study, we analysed the distribution of beta tubulins to detect spindle and cytoplasmic microtubules, alpha acetylated tubulins for sperm microtubules and chromatin configuration in oocytes showing fertilization failure after conventional IVF or intracytoplasmic sperm injection (ICSI). A total of 450 human oocytes that failed to fertilize were studied 20-40 h after IVF or ICSI. In all, 2...

PURPOSE To compare sperm parameters and intracytoplasmic sperm injection (ICSI) outcomes for testicular spermatozoa frozen on the day of the biopsy (DO) with those frozen after 24 h of in vitro culture (D1). METHODS In this retrospective study, from 1999 to 2012, forty-nine azoospermic patients were included to compare sperm (motility and viability) and outcomes (fertilization (FR), implantat...