Hyaluronic acid (HA)-binding is reported to predict the fertilising capacity of spermatozoa, while HA-bound sperm selection reduce incidence miscarriage. However, clinical effectiveness these techniques remains uncertain. This work investigated prognostic value sperm-HA binding (HAB) as a predictor treatment outcomes, and whether HAB-sperm for Invitro fertilisation (IVF)/intracytoplasmic inject...

One important result of egg activation is the establishment of blocks to prevent polyspermic fertilization; these blocks are established on the zona pellucida and the egg plasma membrane. This study examines what the sperm brings to the egg to induce the establishment of the membrane block to polyspermy, building on past evidence that membrane block establishment does not occur in response to p...

The fertility potential of patients with nonobstructive azoospermia (NOA) depends on sperm extraction from the tissue sample and then in vitro fertilization with intracytoplasmic sperm injection (IVF/ICSI). Unfortunately, there is no consensus regarding predictors that can identify nonobstructive azoospermic men with a potentially high yield at the time of sperm extraction. This article analyze...

Sperm nuclear transfer or intracytoplasmic sperm injection (ICSI) is a powerful assisted reproductive technology (ART) for treating human male infertility. Controversial reports of increased birth defects have raised concerns about the ART's safety. The cause for birth defects, however, has remained elusive for analysis in human because of the sample size, male infertility genetics, physiologic...

This study was conducted to determine whether the mode of sperm immobilization prior to intracytoplasmic sperm injection (ICSI) influences fertilization by immature spermatozoa. Of the 837 ICSI cycles evaluated, 81 were performed with epididymal or testicular spermatozoa; 35 cycles with epididymal spermatozoa immobilized in the standard fashion resulted in fertilization and pregnancy rates of 4...

Assisted oocyte activation (AOA) is the technique that simulates the effect of sperm penetration into an oocyte inducing oocyte activation. This technique is used in combination with intracytoplasmic sperm injection (ICSI) to improve the outcome of the fertilization process. It is mostly beneficial and recommended in those cases, when classic ICSI fails. This accounts approximately up to 5 % of...

Fertilisation by intracytoplasmic sperm injection (ICSI), a technique that bypasses the membrane fusion of the gametes, has been widely used to produce offspring in humans and mice. Success with this technique has lent support to the hypothesis that in mammalian fertilisation, a factor from the sperm, the so-called sperm factor, is responsible for oocyte activation and that the fusion process i...

OBJECTIVE To investigate the efficacy of IVF-intracytoplasmic sperm injection (ICSI) in patients who cryobanked semen before cancer treatment. DESIGN Retrospective consecutive study. SETTING University-based IVF unit. PATIENT(S) One hundred eighteen couples undergoing IVF-ICSI using pretreatment frozen sperm. INTERVENTION(S) Treatment follow-up. MAIN OUTCOME MEASURE(S) Semen parameter...

ACKNOWLEDGEMENTS The authors would like to thank Rochford Medical for the kind donation of P-1 throughout the duration of this study. 1. Waterstone, JJ. and Parsons, JH. (1992) A prospective study to Investigate the value of flushing follicles during transvaginal ultrasound-directed follicle aspiration, Fertil. Steril. 57,221-223. 2. Staessen, C., Janssenwillen, C. et al. (1998) Controlled comp...

OBJECTIVE To evaluate the outcomes of patients with moderate oligoasthenozoospermia treated with conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). METHODS A total of 99 couples with moderate oligoasthenozoospermia undergoing their first IVF/ICSI cycle were included in the study. Sibling oocytes were randomized to be inseminated either by conventional IVF o...