Mice have been essential for distinguishing the role of hepcidin in iron homeostasis. Currently, investigators monitor levels of murine hepatic hepcidin-1 mRNA as a surrogate marker for the bioactive hepcidin protein itself. Here, we describe and validate a competitive, enzyme-linked immunosorbent assay that quantifies hepcidin-1 in mouse serum and urine. The assay exhibits a biologically relev...

This indirect method for determination of desferrioxamine (DFO, deferoxamine, Desferal) and its chelated compounds ferrioxamine (FO) and aluminoxamine (AIO) in biological fluids involves metal chelate formation and solvent extraction, the metal being determined by Zeeman atomic absorption spectrometry (Zeeman-AAS). Special attention was paid to the pH and recovery of extraction, iron-aluminum i...

Herein a big Fe-C-Ca cycle, clarifying the basic element flows and energy flows in modern carbon-intensive industries including the metallurgical industry and the cement industry, was proposed for the first time in the contexts of emission reduction and iron ore degradation nowadays. This big cycle was focused on three industrial elements of Fe, C and Ca and thus it mainly comprised three inter...

A simple, selective and highly sensitive extraction method has been developed for the determination of iron(II) spectrophotometrically after solid phase extraction. The absorbance is measured at λmax 644 nm. Beer's law was obeyed up to 450 ng mL(-1) of iron. The molar absorptivity, Sandell's sensitivity, detection and quantification limits were calculated and found to be 1.09×10(5) L mol(-1) cm...

An automated micromethod for measuring iron concentration in serum employing thioglycollic acid and bathophenantroline sulphonate as reducing agent and chromogen respectively, is described. Measurements are carried out using an AutoAnalyzer (Technicon) and require 0.1 ml of sample. The reproducibility, assessed by the mean coefficient of variation (1.9%), and the mean recovery of iron added to ...

Abstract: The huge consumption of iron ores in China has attracted much attention to utilizing low grade complex iron resources, such as high-aluminum hematite-limonite ore, which is a refractory resource and difficult to upgrade by traditional physical concentration processes due to the superfine size and close dissemination of iron minerals with gangue minerals. An innovative technology for a...

The exposure of cells to several metal ions stabilizes HIF-1 alpha protein. However, the molecular mechanisms are not completely understood. They may involve inhibition of hydroxylation by either substitution of iron by metal ions or by iron oxidation in the hydroxylases. Here we provide evidence supporting the latter mechanism. We show that HIF-1 alpha stabilization in human lung epithelial ce...

in this study, we investigated the cardioprotective effects of resveratrol. rats were intraperitoneally administered with resveratrol (25 mg/kg bw) or vehicle (ethanol 10%) for 7 days and their heart subjected to ischemia/reperfusion injury. isolated hearts were langendorff perfused, left ventricular functions as heart rate and developed pressure, as well as, heart antioxidant status were deter...