A rapid and high resolution separation of lanthanides by HPLC technique has been developed using Di-(2-ethylhexyl) phosphoric acid (HDEHP) coated reverse phase column and α-hydroxy isobutyric acid as the complexing reagent for elution. A gradient elution technique has been developed for achieving the separation of the entire lanthanide series. Isocratic elution procedure has also been developed...

We describe a liquid-chromatographic assay for AZT in human plasma and urine. This assay involves the use of two internal standards, allowing reference of AZT peaks to the appropriate internal standard, the choice depending on the range of concentrations encountered. This method is isocratic, specific, sensitive enough to allow quantification of AZT in concentrations observed clinically, and re...

This paper describes the development of a normal-phase liquid chromatography ultraviolet-diode array detection method for the simultaneous quantification of parthenin and coronopilin in the leaves and flowers of Parthenium hysterophorous. The compounds were analyzed on a Merck Si60 silica column (5 µm, 250 × 4 mm) using an isocratic 15:85 mixture of isopropyl alcohol and hexane. The calibration...

A technique is described using ACCELL QMA anion-exchange SEP-PAKs (Waters Associates) with ammonium formate-based solutions, whereby a sample can be processed within minutes to yield resolution of inositol phosphates. Isomers of inositol trisphosphate can then be separated by using this technique in combination with a rapid (5-6 min) isocratic h.p.l.c. procedure. The use of QMA SEP-PAKs offers ...

An isocratic RP-HPLC method was developed for the determination of Cefditoren pivoxil in pharmaceutical formulations using a C-18 column with water-acetonitrile (50:50, v/v) as mobile phase and flow rate 1.2 mL/min (UV detection at 218 nm). Linearity was observed in the concentration range 1.0-250 μg/mL (R2=0.999) with regression equation y=24194x+10749. The forced degradation studies were perf...

This application note describes the use of a quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation procedure. The QuEChERS method employs an extraction and cleanup protocol for the determination of acrylamide with C3-acrylamide as the internal standard. The analyte and internal standard were separated on a reversed phase C-18 column (2.1 mm × 150 mm, 3 μm) by isocratic e...

A simple and precise high-performance liquid chromatographic procedure has been developed for the determination in biological fluids of ciprofloxacin, a new, with extended antibacterial spectrum, quinoline carboxylic acid. The work-up procedure involves a chemical extraction step followed by isocratic chromatography on a reversed-phase analytical column, with ultraviolet detection. The detectio...