pluronic/bile salt/phospholipid mixed micelles (pluronic/bs/ps-mm) drug carrier system for solubilization hydrophobic drugs was developed. a typical hydrophobic compound, pyrene, was selected as a representative hydrophobic compound to model the hydrophobic drugs. five pluronics, f68, f88, f98, f108, and f127 with different ppo chain length were studied. cmc data and solubilization capacities w...

Seaweed is gaining attention as a possible alternative and sustainable source of proteins. This study investigates three protein extraction methods their effect on yield quality when applied to Ulva fenestrata. Two the included alkaline extractions (pH-shifts); one version solubilizing proteins at pH 8.5 them followed by 12 (pH + 12). The third method was mechanical pressing, using double screw...

Experimentation with cultured cells often requires analyzing cellular protein extract by gel electrophoresis and immunoblotting. Traditional methods for extracting cellular proteins by homogenization or detergent solubilization usually produce protein samples that are viscous (due to the presence of DNA) and prone to degradation due to the presence of endogenous protease activity. We have devel...

The serotonin1A (5-HT1A) receptors are members of a superfamily of seven transmembrane domain receptors that couple to G-proteins. They appear to be involved in various behavioural and cognitive functions. This paper reports an efficient strategy to solubilize 5-HT1A receptors from bovine hippocampal membranes using the zwitterionic detergent CHAPS which is mild and non-denaturing. Since high c...

1. The maltase, sucrase, isomaltase and palatinase activities of the chick small intestine are localized in particles that sediment when centrifuged at 100000g for 90min. 2. Solubilization of the particle-bound disaccharidases without loss of activity was achieved by digestion with papain. Trypsin was less effective and caused a preferential solubilization of the sucrase, isomaltase and palatin...

Acid mucopolysaccharides of human plasma have been observed ( 1 ) to vary as do acute phase reactants, i.e., to increase nonspecifically in any inflammatory state. The euglobulin fraction itself with which the acid mucopolysaccharides were associated increased also in such circumstances. On later comparing levels of acid mucopolysaccharides in samples of serum and plasma drawn simultaneously, i...

Nimesulide is a weakly acidic non-steroidal anti-inflammatory drug (NSAIDs). Like many non-steroidal anti-inflammatory drugs, Nimesulide is very sparingly soluble in water (≈ 0.01 mg/mL).The poor aqueous solubility and wettability of Nimesulide gives rise to difficulties in pharmaceutical formulations for oral or parenteral delivery, which may lead to variable bioavailability. Based on the Biop...

BACKGROUND Epidermal growth factor (EGF) plays a fundamental role in the healing of wounds relating to skin damage, the cornea, and the gastrointestinal tract. OBJECTIVES The aim of this study is the cloning, expression, and purification of recombinant human EGF (rhEGF), and an assessment of its activity. MATERIALS AND METHODS In the present experimental study, a synthetic pET28a (+) -hEGF ...