Isogenic variants resistant to alkylating agents have been isolated from the human lymphoblast cell line TK6. The cell lines may be divided into four classes on the basis of resistance to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The sensitive TK6 parental line shows a 37% survival after 45-min exposure to 0.04 microM MNNG; the three classes of more resistant mutants show 37% survival after ...

Background: The study aimed to identify and target effects associated with HORMAD1 expression in breast cancer cells. Materials methods: Isogenic cell line models inducible were used dependencies induced by expression. Results: Expression of HORMAD1, a gene whose function is best understood meiosis, usually restricted germ-line cells but becomes aberrantly expressed 60% triple-negative cancers ...

AIM To characterize AXL receptor tyrosine kinase (AXL) expression in relationship to tumor protein P53 (TP53 gene, p53 protein) and its role in tumor invasion and response to therapy. METHODS We used 14 cell lines, including 3 isogenic pairs carrying mutant/knockout p53, to gain insight into the relationship between AXL and TP53. These included HCT116, HCT116.p53 mutant, RKO, and RKO.p53-/- l...

BACKGROUND Mice lacking the BCL-6 gene product exhibit overexpression of T helper cell type 2 cytokines, and multi-organ inflammatory responses characterized by eosinophilia and infiltration by IgE+ B lymphocytes. Elevated IgE levels appear to result from loss of BCL-6's role in repression of the Stat6-dependent processes of I-epsilon transcription and IgE class-switching. Understanding the reg...

In the stomach, Helicobacter pylori (Hp) adheres to gastric mucous epithelial cells and initiates several different signal transduction events. Alteration of intracellular Ca [Ca]i is an important signaling mechanism in numerous bacteria-host model systems. However, changes in [Ca]i induced by Hp in normal human gastric mucous epithelial cells have not yet been described. In the following studi...

Clustered regularly interspaced short palindromic repeats (CRISPR)-driven traits are commonly introduced into tomato (Solanum lycopersicum) in less than a year. Although CRISPR technology is highly suitable for rapid incorporation of new plants with superior breeding backgrounds, the usefulness these must be demonstrated prior to large-scale distribution any variety possessing them. The jointle...

The brown planthopper (BPH), Nilaparvata lugens Stål, is an insect pest that severely damages rice (Oryza sativa L.) in Asia, causing huge yield loss. Use of resistant variety a cost-effective and eco-friendly strategy for maintaining BPH populations below the economic injury level. However, current have been changed to virulence against varieties. In this study, estimate effective combinations...