Incubation of normal newborn pig aorta with [14C]valine yielded a labeled protein that was isolated and purified with salt-soluble elastin from copper-deficient pig aorta as carrier. The labeled protein is soluble in strong neutral salt solution in the cold. It coacervates reversibly and can be separated into a hydrophobic phase by centrifugation at 32-37”. The protein migrates on polyacrylamid...

OBJECTIVE To examine the safety and efficacy of endovascular management of isolated infrarenal aortic occlusive disease within our centre. DESIGN AND METHODS Retrospective analysis of all patients who underwent endovascular treatment of occlusive disease that is confined to the infrarenal aorta between September 1993 and November 2004. RESULTS Primary aortic stenting was carried out in 16 w...

Lipid emulsions are widely used for the treatment of systemic toxicity that arises from local anesthetics. The goal of this in vitro study was to examine the cellular mechanism associated with the lipid emulsion-mediated attenuation of vasodilation induced by a toxic dose of bupivacaine in isolated endothelium-denuded rat aorta. The effects of lipid emulsion on vasodilation induced by bupivacai...

Interaction of plasma membrane with the cytoskeleton involves a large number of proteins, among them a 36 kDa protein that was found to be involved in the interaction with actin filaments. We have isolated a 36 kDa protein from bovine aorta as a monomer and in a complex with a 10 kDa protein. Partial amino acid sequence determinations show that the 36 kDa and 10 kDa proteins isolated from bovin...

BACKGROUND The effect of ketamine on vasodilation mediated by adenosine triphosphate (ATP)-sensitive K(+) channels has not been studied. The present study was designed to determine whether ketamine might stereoselectively affect vasorelaxation induced by an ATP-sensitive K(+) channel opener in the isolated rat aorta. METHODS Rings of the rat aorta with or without endothelium were suspended fo...

Oscar Langendorff introduced the first method for isolating a heart with contractile activity in 1895. Since then, the Langendorff method has remained a powerful technique in cardiac research and has led to major advances in medicine. The primary goal of the Langendorff method is to provide an isolated heart with oxygen and metabolites via a cannula inserted into the aorta. The Langendorff hear...

In the search for novel chemical scaffolds leading to potential antihypertensive agents, the methanol extract of Murraya paniculata leaves was assessed for its effects on isolated rat aorta rings. The vasorelaxing effect of the chloroform fraction of the methanol plant extract was the most potent for its vasorelaxing activity on rat aorta rings contracted by 60 mM K(+) (K60). Two coumarins were...

Several haloperidol derivatives with a piperidine scaffold that was decorated at the nitrogen atom with different alkyl, benzyl, or substituted benzyl moieties were synthesized at our laboratory to establish a library of compounds with vasodilator activity. Compounds were screened for vasodilatory activity on isolated thoracic aorta rings from rats, and their quantitative structure-activity rel...

OBJECTIVE As in Part I, to elucidate the role of fluid mechanical factors in the localized genesis and development of atherosclerotic lesions in man, here in the abdominal aorta. METHODS Flow patterns and preferred sites of atherosclerotic lesions in the aorta were studied in detail using the same isolated transparent aortic trees prepared from humans postmortem and the flow visualization and...