The concentrations of amikacin and kanamycin were determined in the serum, gallbladder bile, and gallbladder wall of 20 patients undergoing elective cholecystectomy. Of 20 patients, 14 received 500 mg of amikacin intramuscularly and 6 received 500 mg of kanamycin intramuscularly at various times before surgery. In patients receiving kanamycin, detectable levels appeared in bile within 90 min af...

Background. The study of the mechanisms action antibiotics requires integrated use traditional microbiological and physicochemical methods. aim. To response Escherichia coli bacteria to four antibiotics, inhibitors protein synthesis, using combined approach. Methods. Bacteria were grown under aerobic conditions on minimal M9 medium with glucose. Tetracycline, kanamycin, streptomycin chloramphen...

background: staphylococcus aureus can cause infections with a wide spectrum of illnesses ranging from benign skin infections to bloodstream infection leading to mortality. antimicrobial resistance especially methicillin resistance in s. aureus (mrsa strains) is currently problematic. the emergence of mrsa infections has developed in both the healthcare and the community settings.  the aim of th...

Mating experiments have shown that high-level resistance (minimal inhibitory concentration greater than 2,000 microgram/ml) to streptomycin and kanamycin, and resistance to penicillin-streptomycin and penicillin-kanamycin synergism are transferable by conjugation from resistant clinical isolates of enterococci to a sensitive recipient strain. Cesium chloride-ethidium bromide ultracentrifugation...

A rapid, simple, and accurate method for the determination of kanamycin and dibekacin in serum by use of high-pressure liquid chromatography is described. The serum proteins were precipitated with 3.5% perchloric acid containing sodium octanesulfonate. After centrifugation, a sample of the supernatant was directly injected into the chromatograph. The determination of kanamycin and dibekacin was...