نتایج جستجو برای: keywordschicken sybr green rt

تعداد نتایج: 199695  

Journal: :International journal of systematic and evolutionary microbiology 2000
H X Xu Y Kawamura N Li L Zhao T M Li Z Y Li S Shu T Ezaki

A simple and rapid method to determine the G+C content of bacterial chromosomal DNA was developed. It involves determination of Tm by a Light Cycler and calculation of the G+C content by an empirical formula relating Tm to G+C content. Instead of a conventional thermal denaturation method, which monitors the increase of absorbance at 260 nm, thermal denaturation was monitored by the decrease of...

Journal: :Drug metabolism and disposition: the biological fate of chemicals 2017
Joan P Graves Artiom Gruzdev J Alyce Bradbury Laura M DeGraff Matthew L Edin Darryl C Zeldin

The CYP2C subfamily of the cytochrome P450 gene superfamily encodes heme-thiolate proteins that have a myriad of biologic functions. CYP2C proteins detoxify xenobiotics and metabolize endogenous lipids such as arachidonic acid to bioactive eicosanoids. We report new methods and results for the quantitative polymerase reaction (qPCR) analysis for the 15 members of the mouse Cyp2c subfamily (Cyp2...

Journal: :Nucleic acids research 2004
Kyoko Takatsu Toyokazu Yokomaku Shinya Kurata Takahiro Kanagawa

Fluorescence resonance energy transfer (FRET) is a simple procedure for detecting specific DNA sequences, and is therefore used in many fields. However, the cost is relatively high, because FRET-based methods usually require fluorescent probes. We have designed a cost-effective way of using FRET, and developed a novel approach for the genotyping of single nucleotide polymorphisms (SNPs) and all...

Journal: :Canadian journal of microbiology 2011
Jennifer Loveland-Curtze Vanya I Miteva Jean E Brenchley

Standardized procedures must be followed when characterizing, officially describing, and validly naming novel bacteria. For species descriptions, DNA-DNA hybridization still is needed for whole-genome comparisons between close relatives, but many established hybridization methods have drawbacks, such as requiring labeled or large amounts of DNA. We evaluated a new technique based on the spectro...

2016
Jennifer Dang Pedro Mendez Sharon Lee James W. Kim Jun-Hee Yoon Thomas W. Kim Charles J. Sailey David M. Jablons Il-Jin Kim

Next-generation sequencing (NGS) is becoming a standard for genetic analyses of clinical samples. DNAs retrieved from formalin-fixed, paraffin-embedded (FFPE) tissue specimens are commonly degraded, and specimens such as core biopsies are sometimes too small to obtain enough DNA for NGS applications. Thus, it is important to measure both the DNA quantity and quality accurately from clinical sam...

Journal: :Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 2015
Li Juan Li Xue Tian Xiang Juan Kong Xia Chu

A G-quadruplex-based, label-free fluorescence assay was demonstrated for the detection of adenosine triphosphate (ATP). A double-stranded DNA (dsDNA), hybridized by ATP-aptamer and its complementary sequence, was employed as a substrate for ATP binding. SYBR Green I (SG I) was a fluorescent probe and exonuclease III (Exo III) was a nuclease to digest the dsDNA. Consequently, in the absence of A...

Journal: :Nucleic acids research 2003
Steven Giglio Paul T Monis Christopher P Saint

SYBR Green I (SG) is widely used in real-time PCR applications as an intercalating dye and is included in many commercially available kits at undisclosed concentrations. Binding of SG to double-stranded DNA is non-specific and additional testing, such as DNA melting curve analysis, is required to confirm the generation of a specific amplicon. The use of melt curve analysis eliminates the necess...

Journal: :Analytical biochemistry 2002
Jo Vandesompele Anne De Paepe Frank Speleman

Gene expression analysis plays an increasingly important role in many fields of biological research. The recently developed real-time PCR quantification method has many advantages over the conventional quantifications in terms of accuracy, sensitivity, dynamic range, high-throughput capacity, and absence of post-PCR manipulations (1, 2). Sequence-specific fluorescence-labeled probes (e.g., TaqM...

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