The production of D-lactic acid by Lactobacillus lactis RM2-24 was investigated using modified media to increase the efficiency of the fermentation process. The results indicated that the addition of 5 g/l peptone and 1 g/l (NH4)2HPO4 enhanced D-lactic acid production by 32%, as compared to that obtained from non supplemented media, with a productivity of 3.0 g/l/h. Lactate dehydrogenase (LDH) ...

Thermotolerant Bacillus coagulans is considered to be a more promising producer for bio-chemicals, due to its capacity to withstand harsh conditions. Two L-lactate dehydrogenase (LDH) encoding genes (ldhL1 and ldhL2) and one D-LDH encoding gene (ldhD) were annotated from the B. coagulans DSM1 genome. Transcriptional analysis revealed that the expression of ldhL2 was undetectable while the ldhL1...

The oxidation-reduction properties of free cytochrome b2 isolated by controlled proteolysis from flavocytochrome b2, i.e. the flavodehydrogenase-bound cytochrome b2, were investigated by using stopped-flow spectrophotometry. The rapid kinetics of the reduction of cytochrome b2 by flavocytochrome b2 in the presence of L-lactate are reported. The self-exchange rate constant between reduced cytoch...

Rates of formation and dissociation of an abortive ternary complex between lactate dehydrogenase isozymes from various mammalian tissues, NADf, and pyruvate were studied utilizing stopped-flow spectrofluorometry. Formation of the complex was accompanied by a decrease in protein fluorescence at 340 rnp and a loss of lactate dehydrogenase activity. Rates of formation of an abortive lactate dehydr...

Lactate has been shown to be an important oxidative fuel. We aimed to quantify the total lactate oxidation rate (Rox) and its direct vs. indirect (glucose that is gluconeogenically derived from lactate and subsequently oxidized) components (mg·kg(-1)·min(-1)) during rest and exercise in humans. We also investigated the effects of endurance training, exercise intensity, and blood lactate concent...

introduction: in some intoxicated patients, cerebrospinal fluid (csf) is examined due to the prolonged loss of consciousness, focal neurologic findings, and fever of unknown origin. we aimed to evaluate the probable relationship between the different toxicity causes and the csf indices in poisoned patients and determine if they could predict the patients’ outcome. methods: all patients who had ...

background tracking various biomarkers in serum, gingival crevicular fluid (gcf), and saliva has been introduced as a diagnostic tool for periodontal disease detection. objectives the aim of this study was to compare salivary lactate dehydrogenase (ldh) levels in subjects with periodontal disease and levels in subjects without periodontal disease. conclusions salivary ldh levels can be used as ...

in the present study, changes of values of serum 17ß-estradiol (e2), asparate amino teransferase (ast), alanine amino transferase (alt) and lactate dehydrogenase (ldh) following intraperitoneal (i.p) injection with the aromatase inhibitor (ai) letrozole were investigated in rainbow trout (oncorhynchus mykiss). forty- eight apparently healthy rainbow trout (mean weight of 845 g) divided in four ...

Membrane-bound L-lactate dehydrogenase was solubilized from the membranes ofEscherichia coli and purified to homogeneity using conventional procedures. The enzyme had a pH optimum of 8 to 9 and was specific for L-lactate. Its apparent K, for L-lactate and maximal velocity were 1.2 x lo-’ M and 31 pmol of tetrazolium dye reducedlminlmg of protein, respectively. It had a polypeptide molecular wei...

Lactate is an abundant metabolite, produced by host tissues and commensal organisms, and it represents an important potential carbon source for bacterial pathogens. In the case of Neisseria spp., the importance of the lactate permease in colonization of the host has been demonstrated, but there have been few studies of lactate metabolism in pathogenic Neisseria in the postgenomic era. We descri...