Extracting fungal mRNA from ectomycorrhizas (ECMs) and forest soil samples for monitoring in situ metabolic activities is a significant challenge when studying the role of ECMs in biogeochemical cycles. A robust, simple, rapid, and effective method was developed for extracting RNA from rhizospheric soil and ECMs by adapting previous grinding and lysis methods. The quality and yield of the extra...

Specific rDNA ITS amplifications, microsatellite-primed PCR and ITS-SSCP analysis were applied to identify and characterize pre-selected isolates of the edible ectomycorrhizal fungus Lactarius deliciosus in different stages of the life cycle. Sampling was performed from pure cultures, mycorrhizas and soil from experimental plots established with nursery-inoculated pine seedlings. A newly-design...

Plants with therapeutic potential are a rich resource for the inhabitants of country, Romania among them, having diverse flora not only from plants but also fungi. New trends in biopharmaceuticals addressed studies related to topical administration, representing great advantage; research has determined biological activities vivo and vitro. This study aimed present main active compounds extracts...

En un vivero forestal, a la planta se le brindan los cuidados e insumos necesarios para favorecer su calidad; con finalidad de que tenga mejores oportunidades durante desarrollo inicial. El objetivo del presente estudio fue evaluar supervivencia y crecimiento en diámetro altura Pinus engelmannii una reforestación, base incorporación inoculantes micorrícicos comerciales etapa preacondicionamient...