Cysteine-conjugated antibody-drug conjugates (ADCs) are manufactured using controlled partial reduction and conjugation chemistry with drug payloads that typically occur in intervals of 0, 2, 4, 6, and 8. Control of heterogeneity is of particular importance to the quality of ADC product because drug loading and distribution can affect the safety and efficacy of the ADC. Liquid chromatography ul...

BACKGROUND Migrant men who have sex with men (MSM) have a higher predisposition for HIV transmission. We aimed to determine and compare the prevalence and trends of HIV, Syphilis, and HCV between migrant and resident MSM in Shandong, China. METHODS A serial cross-sectional study was conducted in eight cities in Shandong, China from 2010 to 2014. The surveys collected information on demographi...

Two analytical platforms, gas chromatography (GC) coupled to quadrupole-time-of-flight (QTOF) mass spectrometry (MS) and reversed-phase ultrahigh performance liquid chromatography (UHPLC) coupled to diode array (DAD) and QTOF detection, were applied in order to study the alkaloid profile of Fumaria capreolata. The use of these mass analyzers enabled tentatively identifying the alkaloids by matc...

In this paper we analyse static properties of mass spring models (MSMs) with the focus modelling non crystalline materials, and explore basic improvements, which can be made to MSMs disordered point placement. Presented techniques address problem high variance MSM occur due randomised nature distribution. The is placed on tuning parameters in a way would compensate for local non-uniformity dens...

Over the last 20 years in Pfalz region of Germany, GDD were highest vintages 2018-2020. To visualize effect climate change for development TSS and TAwere documented during Pinot noir grape ripening 1998-2021. It was observed that causes sooner accelerated ripening, however, or not connected with each other. The presented study investigated impact on berry physiology development, heterogeneity c...

CONSPECTUS: Protein function is inextricably linked to protein dynamics. As we move from a static structural picture to a dynamic ensemble view of protein structure and function, novel computational paradigms are required for observing and understanding conformational dynamics of proteins and its functional implications. In principle, molecular dynamics simulations can provide the time evolutio...