A new liquid culture medium prepared with chemicals that can be obtained economically and commercially was tested in in vitro cultivation of Leishmania promastigotes to obtain a large number of organisms to use in serological studies. The number of Leishmania infantum and Leishmania tropica promastigotes taken from Novy-MacNeal-Nicolle (NNN) medium reached 1 x 10(7)/ml at the end of the 8th day...

A estrutura dos genes ribossômicos de Leishmania foi caracterizada, através da determina­ ção de seus padrões de restrição, com o objetivo de descrever regiões do gene que permitam a identifica­ ção destes organismos. A análise comparativa dos padrões de restrição de seis espécies de Leishmania permitiu identificar heterogeneidade intra e interespecífica na organização das regiões espaçadoras d...

cutaneous leishmaniasis is one of the most important parasitic diseases, which are endemic in different parts of iran.   leishmania major and l. tropica are the primary causative agents of this disease. the aim of the present study was to detect the multiple forms of l. major in lung. ppromastigotes of l. major at stationary phase were injected to balb/c mice. after 60 days, the different forms...

Background and Aims: The protozoan parasites of the genus Leishmania are the causative agents of various clinical diseases. Different methods of cultivation of Leishmania parasites are available. In the present study, the efficacy of the LB broth with rabbit lyophilized anti-sheep red blood cell haemolysin was evaluated in the cultivation of promastigotes of Leishmania major. Materials and Met...

Background: Cationic immune stimulating complexes (PLUSCOMs) are particulate antigen delivery systems. PLUSCOMs consist of cationic immunostimulatory complexes (ISCOMs) derivatives and are able to elicit in vivo T cell responses against an antigen. Objective: To evaluate the effects of PLUSCOMs containing Leishmaniamajor antigens (SLA) on the type of immune response generated in the murine mod...

Infection of dendritic cells by the human protozoal parasite Leishmania is part of its survival strategy. The dendritic cell receptors for Leishmania have not been established and might differ in their interactions among Leishmania species and infective stages. We present evidence that the surface C-type lectin DC-SIGN (CD 209) is a receptor for promastigote and amastigote infective stages from...

background pentavalent antimonials remain the choice of treatment for leishmaniasis, despite their toxicity, high cost, and difficult administration. as an alternative, morphine may induce the healing process of cutaneous leishmaniasis by its immunoregulatory characteristics. objectives to study the effect of morphine on the wound-healing process of cutaneous leishmaniasis (cl) in a mouse model...

background and objectives: several natural compounds have been identified for the treatment ofleishmaniasis. due to a few safe drugs and the side effects caused by available chemotherapy, some new drugs for treatment of leishmaniasis are requested.  the genus pulicaria (asteraceae) is represented in the flora of iran by five species. phytochemical studies on pulicaria species have revealed some...