BACKGROUND & OBJECTIVES Due to emergence of drug resistance in Mycobacterium tuberculosis, there is a need to have accurate and rapid methods for detection of drug resistance to important drugs like rifampicin. The present study was aimed at evaluation of a commercially available INNO-LiPA assay, for the detection of mutation in rpoB gene region of M. tuberculosis and correlate these mutations ...

Metagenomic analyses have advanced our understanding of ecological microbial diversity, but to what extent can metagenomic data be used to predict the metabolic capacity of difficult-to-study organisms and their abiotic environmental interactions? We tackle this question, using a comparative genomic approach, by considering the molecular basis of aerobiosis within archaea. Lipoylation, the cova...

Two methods for genotyping hepatitis C virus (DNA enzyme immunoassay [DEIA] and line probe assay [Inno-LiPA HCV I and II]) were compared on 120 samples and of these 87% were assigned to the same subtype by both assays. There were 15 subtyping discrepancies which involved 5% of type 1 isolates and 90% of type 2 isolates. Amplified products from the core and 5' untranslated regions (UTR) were seq...

Abstract Funding Acknowledgements Type of funding sources: Public grant(s) – National budget only. Main source(s): Slovenian Research Agency Background Despite high levels sedentary behaviour (SB) in patients with coronary artery disease (CAD), there is still limited evidence on the efficacy multimodal exercise interventions combined physical activity (PA) counselling SB and PA cardiac rehabili...

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SAEPULLOH, M and ROVIRA H.G. 2003. Isolation and identification of infectious laryngotracheitis virus from outbreaks at Lipa City, Batangas, Philippines. JITV 8(2): 122-134. Infectious laryngotracheitis (ILT) is an acute, highly contagious respiratory disease of poultry characterized by respiratory disorder such as coughing with blood exudate from the trachea. The disease is caused by Herpesvir...

Pseudomonas alcaligenes M-1 secretes an alkaline lipase, which has excellent characteristics for the removal of fatty stains under modern washing conditions. A fed-batch fermentation process based on the secretion of the alkaline lipase from P. alcaligenes was developed. Due to the inability of P. alcaligenes to grow on glucose, citric acid and soybean oil were applied as substrates in the batc...

BACKGROUND Human papillomavirus (HPV) infection causes cervical cancer, thus necessitating early detection by screening. Rapid and accurate HPV genotyping is crucial both for the assessment of patients with HPV infection and for surveillance studies. METHODS Fifty-eight cervicovaginal samples were tested for HPV genotypes using four methods in parallel: nested-PCR followed by conventional seq...

In the last few years, a variety of DNA-based human leukocyte antigen (HLA) typing methods have emerged, revealing the extreme polymorphism of HLA genes. This polymorphism makes it difficult for a clinical laboratory to establish the best HLA typing strategy. In this study we have compared two techniques for performing HLA-DRB typing: a commercial rapid assay based on the polymerase chain react...

Background and Aims: Hepatitis B virus (HBV) infects the liver and causes acute and chronic hepatitis, hepatocellular carcinoma and cirrhosis. HBV has been divided to eight genotypes (A–H) and subgenotypes of A, B, C and F. For the first time, we determined HBV genotypes in infected samples by INNO-LiPA method in Yazd, central province of Iran. Materials and Methods: This study was performed...