Screening of lipolytic strains using Rhodamine-B/olive oil plate technique allowed the selection of Aspergillus niger MYA 135. Lipase production in submerged culture containing 2 % olive oil was enhanced by more than 50 % compared to basal cultural conditions. Optimal catalytic conditions for olive oil-induced lipase were pH=6.5 and 30–35 °C. These values were shifted to the acid region (4.0–6....

Catecholamine-induced lipolysis in isolated human adipocytes during the first year of life was investigated. During this period fat cell size increased markedly. Basal and catecholamine-induced glycerol release were positively correlated with age when lipolysis was expressed per cell. However, when lipolysis was expressed per unit of cell surface area (micrometer squared), this correlation was ...

Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored ...

The crude enzyme fraction was isolated from the fresh unripe fruits of Momordica charantia Linn and screened for its amylolytic and lipolytic activity according to standard literatures. The results indicated that the crude enzyme fraction is having effective amylolytic and lipolytic activities. These enzymatic activitieis of the extract substantiate the validity of claims made in traditional sy...

Influence of storage temperature on the shelf life of the pasteurized milk and the enzymatic activity of psychrotrophic bacteria in whole and skimmed pasteurized milk were the main goals of the presented work. It was demonstrated that the storage temperature of 10C reduces the shelf life of pasteurized milk to one third in comparison with a storage at 4 C. The average shelf life of the full cre...

Adipose tissue contains low-molecular-weight soluble substances which are utilized in preference to lipid for bacterial growth. These components are present in low concentration at the surface of adipose tissue, and the pH of the surface is high (greater than 7.0). Bacteria growing on a thin layer of agar over an adipose tissue surface utilized glucose preferentially, but this was soon exhauste...

Microbial enumeration and identification were carried out on several oil contaminated soil samples collected from gasoline and diesel stations. Bacteria were the most dominant microbiota and were therefore classified to generic level. Eleven main genera were detected and Corynebacterium was the predominant genus in all the samples. Biochemical characterisation and substrate utilisation showed h...

The lipase producing bacteria were screened from the soil both qualitatively by Tween 80, rhodamine B agar assays and quantitatively by titrimetry with the objective of finding a bacterial strain capable of producing an alkaline lipase. The higher lipolytic activity of 17.43 U/ml was recorded for the isolate L13. Traditional methods viz. morphological, cultural and biochemical characteristics w...