Experiments were carried out to study the role played by the extrahepatic and hepatic lipolytic enzymes in lipoprotein catabolism. Chylomicra and very low density lipoproteins containing [I-“Hlglyceryl triacylglycerols radiolabeled in uiuo were incubated with purified milk lipoprotein lipase to produce lipoprotein remnants rich in monoacylglycerol. The primary products of the milk lipoprotein l...

To elucidate mechanisms responsible for the increased plasma triglyceride (TG) which occurs in diet-induced hypercholesterolemia in rabbits, albino rabbits were fed a 3% cholesterol diet for various durations, and the following determinations were carried out: plasma lipid levels; turnover rate of plasma TG (labeled very low density lipoprotein, or glycerol-2-H method); lipoprotein lipase activ...

In normal subjects, apolipoprotein E (apo E) is present on very low density lipoproteins (VLDL) (fraction I) and on particles of a size intermediate between VLDL and low density lipoproteins (LDL) (fraction II). The major portion of apo E is, however, on particles smaller than LDL but larger than the average high density lipoproteins (HDL) (fraction III). To investigate the possible role of the...

Previously we demonstrated a marked sex difference in the metabolism of chylomicrons in adult rats. In males, radiolabeled chylomicrons displayed a longer dwell time on endothelial surfaces, which resulted in a decreased chylomicron uptake by the liver. The increased rate of chylomicron metabolism in females was associated with increased postheparin lipoprotein lipase activity. In the present s...

Alimentary triglycerides are transported in the blood primarily in the form of emulsified particles called chylomicrons, which consist predominantly of triglycerides, but also contain a small proportion of cholesterol, phospholipid, and protein (1). Recently, Rodbell and Fredrickson (2) have found that one of the proteins associated with chylomicrons appears to be the same as the protein isolat...

Chimeric molecules between human lipoprotein lipase (LPL) and rat hepatic lipase (HL) were used to identify structural elements responsible for functional differences. Based on the close sequence homology with pancreatic lipase, both LPL and HL are believed to have a two-domain structure composed of an aminoterminal (NHz-terminal) domain containing the catalytic Ser-His-Asp triad and a smaller ...

Adipose tissue lipoprotein lipase (LPL) is the enzyme responsible for hydrolysis of circulating lipoprotein triacylglycerol (TAG). Successive passages of very-low-density lipoprotein (VLDL) through adipose tissue are thought to produce progressive delipidation of the particles, with the eventual formation of intermediate-density lipoprotein (IDL) and low-density lipoprotein (LDL) [ 1 I . The hy...

1. Post-heparin lipolytic activity in man has been studied by using a triglyceride substrate emulsion containing different emulsifiers. 2. The lipolytic activity measured was profoundly influenced by the type of emulsifier used in the substrate. Substrate stabilized by synthetic emulsifiers give higher lipolytic activity than Intralipid, which contains egg phospholipids as emulsifiers. This dif...

Abhrak bhasma is a commonly used ayurvedic drug against many diseases including hepatitis. It is tested in albino rats using a model of hepatitis induced by a single dose of CCl4 (3 ml/kg body wt). Different doses of abhrak bhasma (10, 20, 30 and 40 mg/kg body wt) were tested to decide the dose related hepatoprotective efficacy. The centrolobular necrosis induced by single dose of CCl4 was redu...