the present work examines leaching of lipoxygenase-1 from soybean flour employing stirred tank vessel. the effect of operating parameters, impellers speed, operational period, temperature, ph and scale-up were considered.the acetic acid ph: 5.2 increased the leaching of lipoxygenase- 1.the sensitivity of agitator speed and geometrical-scale-up on the enzymeleaching has been conducted. the effec...

A new potato tuber lipoxygenase full-length cDNA sequence (lox1:St:2) has been isolated from potato tubers and used to express in Escherichia coli and characterize a novel recombinant lipoxygenase (potato 13/9-lipoxygenase). Like most plant lipoxygenases it produced carbonyl compounds from linoleate (the preferred substrate) and was purified in the Fe(II) (ferrous) state. Typical of other potat...

A widespread range of oxidative coupler reactions have been approved for drugs. Majority these reactionsare color producing reactions.Generally, spectrophotometric procedures are easy to achieve, little-cost, sensitive and selective, howevereach way has its advantages weaknesses, which an essentialfeature whenchoosing the technique estimation drugs.So, purpose objective in this paper is review ...

Lipoxygenases are non-heme iron dioxygenases that catalyze the oxygenation of polyunsaturated fatty acids. Using soybean lipoxygenase-1 as a model, we have shown that two classes of lipoxygenase inhibitors currently in development as potential antiinflammatory agents obtain a significant amount of their potency by reducing the lipoxygenase active-site iron from the active ferric state to the in...

The potential of the lipoxygenase from Gaeumannomyces graminis to remove lipophilic extractives from eucalypt and flax pulps was investigated. Pulp treatments were performed with the lipoxygenase both in the presence and absence of linoleic acid, and were followed by a peroxide bleaching stage. The main lipophilic extractives from eucalypt pulp such as conjugated and free sterols decreased up t...

UNLABELLED Ten different varieties of tomatoes were separated into peel and flesh and each portion was measured separately. Headspace volatiles were measured in real time using selected ion flow tube mass spectrometry. Lipoxygenase activity was measured using the adsorption of conjugated dienes formed by lipoxygenase. Lipid was extracted and fatty acids were quantified using a gas chromatograph...

Exposure of soybean (Glycine max) seedlings to low levels of atmospheric methyl jasmonate induced the expression and accumulation of one or more lipoxygenase(s) in the primary leaves, hypocotyls, epicotyls, and cotyledons. In the primary leaf, the major site of lipoxygenase accumulation in response to methyl jasmonate was in the vacuoles of paraveinal mesophyll cells. In the other organs, howev...

The enzyme soybean lipoxygenase (SLO) has served as a prototype for hydrogen-tunneling reactions, as a result of its unusual kinetic isotope effects (KIEs) and their temperature dependencies. Using a synergy of kinetic, structural, and theoretical studies, we show how the interplay between donor-acceptor distance and active-site flexibility leads to catalytic behavior previously predicted by qu...

Enzymatic reactions taking place in minimally processed vegetables are considered as a major problem, because they adversely affect sensorial and nutritional quality. Polyphenol oxidase (PPO), peroxidase (POD) and lipoxygenase (LOX) from lettuce were purified on a column packed with positively charged diethylaminoethyl (DEAE) cellulose by applying pH gradient elution from pH=4.0 to 9.0. The mai...