Liquid chromatography in open tubes is evaluated for applicability to clinical analysis. The principal advantages of this technique include high separation power, reduced solvent consumption, and small sample volume requirements. Technology for the production of columns as small as 8 micron (i.d.) is described. Preliminary results are encouraging and, with further development in column and dete...

this article describes a simple and fast high-performance liquid chromatography method for the determination of methotrexate (mtx) in plasma. samples were collocted from children receiving high-dose mtx at shafa hospital (ahvaz university of medical sciences, ahvaz, iran) at various times after the end of eachinfusion. plasma was deproteinized with trichloroacetic acid and the supernatant was i...

an accurate and sensitive reversed-phase high-performance liquid chromatograph-ic method for determination of sertraline in human serum is described using 4-chloro-7-nitrobenzofurazan as pre-column derivatization agent. the drug and an internal standard (azithrimycin) were extracted from serum using a mixture of diethyl ether-chloroform and subjected to the pre-column derivatization with the re...

Ishii’s group at Nagoya University started microcolumn liquid chromatography (μLC) more than 30 years ago [1]. His ideas have been realized in user−friendly μLC systems which are now commercially available from a number of manufacturers. In the meantime, injection valves with low dead volume, pumping systems working at low flow rates, high−resolution columns, and novel detection systems have be...

The high-pressure liquid chromatographic separation of ail of the known metabolites of vitamin D2 and vitamin D3 found in biological fluids has been achieved. This technique has been successfully applied to the analysis of vitamin D mixtures, purification of vitamin D metabolites, and identification of radioactive peaks. Some theoretical bases for the observed resolutions are suggested. Supplem...

Edström, L. 2014. Adsorption Studies with Liquid Chromatography. Experimental Preparations for Thorough Determination of Adsorption Data. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 1118. 56 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-8858-1. Analytical chemistry is a field with a vast variety of applications. A robust com...

A h igh-per formance l i q u i d chromatography (HPLC) method has been deve loped f o r t h e s e p a r a t i o n of o l e f i n , s a t u r a t e , and a r o m a t i c hydrocarbons found i n h i g h b o i l i n g d i s t i l l a t e s and r e s i d u e s of s h a l e o i l and i n whole s h a l e o i l s . The dual-column chromatographic system uses s i l i c a g e l i n one column and s i l i...

A novel liquid chromatographic system which enables high temperature ultrafast liquid chromatography (HTU-FLC) has been designed through the careful consideration of heat transfer, band broadening, and pressure drop. Studies of the effect of linear velocity on the HETP show that column efficiency at high velocity, especially of well-retained solutes, dramatically improves at higher temperatures...

Modern chromatography by virtue offantastic capabilities for separation, qharacterization, quantitation and fractionation has emerged as a potential analytical technique for hydrocarbon industry. Presently the gas chromatography alongwith high performance liquid chromatography coverwide range ofapplications in diversified areas including Basic and applied research. Availability of large number ...

Identification of routine mycobacterial isolates by gas-liquid chromatography profile analysis was performed on 335 strains received at the Mayo Clinic over a 10-month period. Comparison of identification by gas-liquid chromatography versus conventional biochemical profiles was made. The two methods agreed on the identification of 320 isolates, with gas-liquid chromatography profiling making ei...