The effect of 24 anti-amoebic and other chemotherapeutic compounds on six strains of hartmannellid amoebae was studied by tissue culture, agar diffusion, and liquid axenic culture. Of the recognized anti-amoebic compounds only one inhibited growth of the amoebae, which were remarkable for their resistance. Two compounds, hydroxystilbamidine isethionate and 5-fluorocytosine, showed some amoebici...

Spent culture supernatant from early-stationary-phase Mycobacterium tuberculosis H37Ra cultures increased the viability of bacilli from aged cultures of this strain and allowed small inocula to initiate growth in liquid culture. The resuscitation factor was acid labile and heat stable, with a mass of less than 1,375 Da.

Visibility of the Deer Fly in Flight: Elek Ludvigh...... 176 Production of Yellow Bean Mosaic in Beans by Virus From Mottled Gladiolus: F. P. McWhorter, Lytton Boyle, and B. F. Dana ........................... 177 Production and Treatment of Hepatitis With Focal Necrosis of the Liver in White Mice: Paul Engel. 178 Forty-Five Years of Continuous Cropping With Lima Beans: Francis L. Smith, A. H. ...

Budding yeasts are highly suitable for aging studies, because the number of bud scars (stage) proportionally correlates with age. Its maximum stages are known to reach at 20-30 stages on an isolated agar medium. However, their stage dynamics in a liquid culture is virtually unknown. We investigate the population dynamics by counting scars in each cell. Here one cell division produces one new ce...

Use of the air-liquid interface culture technique has produced improved morphological differentiation of rodent, canine, and human tracheal epithelia. We have investigated the effect of this culture technique on ion transport activities of cultured canine bronchial epithelia. These cells were isolated from excised airways by enzymatic digestion and plated on permeable collagen membrane substrat...

A recently developed liquid culture technique was used to determine the proliferative and maturational character istics of leukemia cells from 7 patients with chronic myelogenous leukemia (CML). Marrow and blood leukocytes were cultured in an in vitro diffusion chamber for up to 45 days. Proliferation was assessed by viable cell counts and thymidine-3H-labeling indices. Maturation was determined

We have developed a technique that simplifies the process of screening large quantities of Arabidopsis thaliana seeds to identify antibiotic-resistant individuals. We use this technique in our laboratory for screening seed populations after transforming by vacuum infiltration (1). Conventional screening methods are materialand labor-intensive, particularly if transformation frequencies are low,...

Armillaria sp. F022, a white-rot fungus isolated from decayed wood in tropical rain forest was used to biodegrade anthracene in cultured medium. The percentage of anthracene removal by Armillaria sp. F022 reached 13 % after 7 days and at the end of the experiment, anthracene removal level was at 87 %. The anthracene removal through sorption and transformation was investigated. 69 % of eliminate...

A key step in the process of isolating microbial natural products is preparation an extract from a culture. This determines which molecules will be available for detection subsequent chemical and biological analysis biodiscovery pipeline. In present study we wanted to document potential differences performance between liquid–liquid extraction using ethyl acetate liquid–solid poly-benzyl-resin. ...

In 2005 a Mycobacterium genavense infection was diagnosed in one blue-headed parrot (Pionus menstruus) imported from Surinam (South America), the first such incidence in the Czech Republic. The bird died suddenly. Histopathological examination revealed a diffuse granulomatous inflammatory reaction in the intestinal mucosa. Tissue sections of the intestines stained by Ziehl-Neelsen were microsco...