Murine fetal liver cells have been fractionated by fluorescence-activated cell sorting into two fractions termed. for convenience, the pre-CFC and CFC (colony-forming cell) fractions. which differ in their relative binding of the lectin. pokeweed mitogen. The CFC fraction contained a high frequency of CFC (26%) and in liquid cultures stimulated with colony-stimulating factors generated large nu...

A colony-stimulating factor (CSF) that stimulates the in vitro proliferation of human and murine bone marrow cells was purified from normal human urine to apparent electrophoretic homogeneity (CSFHu 5). The nature of its stimulating activity on colony formation was determined by using unfractionated and monocyte-depleted bone marrow cells from humans and mice. The factor was able to stimulate c...

The white rot fungus Phanerochaete chrysosporium metabolized phenanthrene when it was grown for 7 days at 37 degrees C in a medium containing malt extract, D-glucose, D-maltose, yeast extract, and Tween 80. After cultures were grown with [9-14C]phenanthrene, radioactive metabolites were extracted from the medium with ethyl acetate, separated by high-performance liquid chromatography, and detect...

The long-term suspension growth of normal, immature myeloid cells from fresh human cord blood was recently reported and required cells separated on supplemented discontinuous Percoll gradients, growth in media containing hydrocortisone and vitamins D3, and gentle, continuous agitation (13). When normal adult bone marrow (six donors) or blood from Epstein-Barr virus (EBV)-seropositive donors (ni...

Food fermentation using lactic acid bacteria (LAB) is an ancient technique that has been deemed a simple and economical way to modify nutritional contents of plant-based foods. In many cultures, this practice shows long history with wide variety fermented liquid semi-liquid traditional foods being produced from cereals, legumes, tubers. Nutritionally relevant benefits the process are becoming i...

Immobilized bacteria are being assessed by industry for drug delivery, novel fermentation systems and the protection of organisms in harsh environments. Alginate bioreactors containing Streptomyces nodosus were examined for community structure, cell viability and amphotericin production under different growth conditions. When cell proliferation was encouraged, substrate hyphae were found inside...