We describe a routine method for determining plasma concentrations of quinidine by liquid chromatography. The procedure requires 1.0 ml of plasma (or serum) and involves internal standard addition, extraction with ether, and separation on a column of microparticulate silica. Day-to-day CV (15 days) was less than 5% and no deterioration in column performance has been observed during 12 months. C...

Sulfonamides were extracted from honey with acetate buffer (pH 5.0), and cleaned up by solid phase extraction (SPE) procedure. The liquid chromatography (LC) separation was carried out on RP C18 column using acetic acid and acetonitrile as the mobile phase in gradient mode and monitored with fluorescence detector, after pre-column derivatisation. Recoveries from spiked honey were above 80%, and...

A comprehensive study pertaining to the emulsion liquid membrane (ELM) extraction process to enrich dilute aqueous solutions of silver salt is presented. The study has highlighted the importance and influence of membrane composition for maximizing the extraction of Ag+ ions. The liquid membrane was made up of Cyanex-302 as an extractant and the industrial solvent mainly consists of p...

In this study, we combined Ionic Liquid-based Dispersive Liquid Liquid Micro Extraction (IL-DLLME) with FAAS for determining the palladium in different real samples at the trace level. 1-hexyl-3-methylimidazolium hexafluorophosphate [Hmim][PF6] ionic liquid and 1-(2-pyridylazo) 2-naphthol (PAN), were chosen as the extraction solvent and the chelating agent, respectively. The hydr...

Instability of capillary wave and breakup of a square cylindrical liquid column during its relaxation have been investigated numerically by simulating three-dimensional Navier-Stokes equations. For this investigation a computer code based on volume-of-fluid (VOF) method has been developed and validated with published experimental results. The result shows that the agreement of numerical simulat...

A procedure is described that uses high-pressure liquid chromatography for assay of gentamicin in serum. The technique involves extraction of gentamicin from serum by using a CM-Sephadex column and analysis by reverse-phase, ion-pair chromatography. Continuous-flow, post-column derivatization with o-phthaladehyde is used to form fluorescent products for detection. The possible scope of this met...

Oxytetracycline (OXY) is a broad-range antimicrobial routinely used in pig production, at doses in the range of few g/kg of medicated feed, during the weaning period. It could persist at ppm level in pig liquid manure that routinely is used for organic fertilisation. In the present work we describe a methodology to study OXY environmental fate in arable land where crops are cultivated for anima...

Knowledge of droplet behavior is one of the most important criteria for determination of mass transfer kinetics for choosing the type of liquid-liquid extraction columns. Mean drop size data of dispersed phase droplets in continuous phase have been obtained for Various Rotary Agitated Liquid-Liquid Extraction Columns . The effects of operational variables such as mass transfer directions with a...

A high performance liquid chromatography method for determining the levels of 4-pregnene-3-ones in tissue containing fat, e.g. in thymus is reported. Following the extraction with chloroform/methanol the fat has been separated from the steroidal-fraction by using disposable extraction-columns. The steroidal-fraction has been analysed in two separation systems, on a RP18-column with a methanol/w...