The performance characteristics of the Tandem-MP Ostase assay, a new microplate immunoassay for bone-specific alkaline phosphatase (bone ALP; EC 3.1.3.1) in human sera, are described. Bone ALP is bound to streptavidin-coated microwells by a single biotinylated anti-bone ALP monoclonal antibody. Antigen is detected by the addition of p-nitrophenyl phosphate. The assay is performed at room temper...

Background: This study was conducted to evaluate the effects of feeding on biochemical parameters in terms metabolic profile female and male lambs Dorper Lacaune breeds at developmental stages from birth. Methods: A total 32 singleton newborn breed (n= 8 for each group; female) were used. The trial continued 5 nutritional periods 21-day after first two weeks (0-14 days) suckling period. determi...

Purpose: To investigate the effect of miR-595 on osteogenic differentiation bone marrow mesenchymal stem cells (BMSCs).Methods: Human BMSCs were osteogenically differentiated, and protein expression alkaline phosphatase (ALP), osteocalcin (OCN), Runt-related transcription factor 2 (RUNX2) evaluated by western blot. Expression was measured quantitative reverse (qRT-PCR). The viability determined...

Fluorescent probe 1, showing a high fluorescence turn-on signal ratio, enables the real-time imaging of endogenous alkaline phosphatase (ALP) activity in living cells, and the fast and quantitative analysis of enzyme activity at the single-cell level.

In this present study, we fabricated an LSI-based amperometric chip device with highly temporal and spacial resolution for electrochemical imaging, applied to detection of alkaline phosphatase (ALP) activity and respiration activity of embryonic stem (ES) cells.

OBJECTIVE The semi-quantitative method of bone scintigraphy [bone to soft tissue (B/ST) ratio] has been used in diagnosing and evaluating systemic metabolic bone diseases. The aim of this study is to evaluate of the therapeutic effect of secondary hyperparathyroidism (SHP). METHODS The subjects were ten hemodialysis patients with SHP. Seven patients underwent parathyroidectomy (PTX), and 22-O...

Human gingival (HG), periodontal ligament (HPL) and alveolar bone (HAB) cells (first subculture) were cultured (10(4) cells/cm2) for 35 days in alpha-Minimal Essential Medium supplemented with 10% fetal bovine serum in the presence of (i) ascorbic acid (AA, 50 microg/mL), (ii) AA + beta-glycerophosphate (betaGP, 10 mM) and (iii) AA + betaGP + dexamethasone (Dex, 10 nM). Cultures were assessed f...

The unexpected enhancement of alkalinee phosphatase (ALP) activity in the serum of a group of STZ-diabetic rats recieving the Teucrium polium extract (with hypoglycaemic property) may be attributed to its probable side effects. In order to get a more precise knowledge about this subject, we evaluated the effect of T. polium extract on the plasma membrane ALP activity of K562 cell line. Our data...

S49 mouse lymphoma cells undergo apoptosis in response to the ALP (alkyl-lysophospholipid) edelfosine (1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine), FasL (Fas ligand) and DNA damage. S49 cells made resistant to ALP (S49(AR)) are defective in sphingomyelin synthesis and ALP uptake, and also have acquired resistance to FasL and DNA damage. However, these cells can be re-sensitized follo...