As recently shown, ultramicroscopy (UM) allows 3D-visualization of even large microscopic structures with microm resolution. Thus, it can be applied to anatomical studies of numerous biological and medical specimens. We reconstructed the three-dimensional architecture of tomato-lectin (Lycopersicon esculentum) stained vascular networks by UM in whole mouse organs. The topology of filigree branc...

Young tomato plants (Lycopersicon esculentum Miller, cultivar Rutgers) grown in solution culture at 27 degrees at 2 light intensities with adequate boron (0.1 mg/l) and treated with these 2 intensities in the absence of adequate boron developed root boron deficiency symptoms. The typical deficiency symptoms of decreased root elongation, increased depth of brown color and decreased RNA content o...

Streptomycin enhances the synthesis of anthocyanins and inhibits the synthesis of chlorophylls and the development of chloroplasts in dark-grown seedlings of cabbage (Brassica oleracea), mustard (Sinapis alba), tomato (Lycopersicon esculentum), and turnip (Brassica rapa) exposed to prolonged periods of irradiation in various spectral regions. These results suggest that the contribution of photo...

Non-grafted tomato plants (Lycopersicon esculentum L. cv. Tmknvf2) and grafted tomato plants (L.esculentum L. cv. Tmknvf2¿L.esculentum L. cv. RX-335) were grown for 30 days at three different temperatures (10æC, 25æC and 35æC). In the leaves of these plants, the enzymatic activities of SOD, GPX, CAT, APX, DHAR and GR were analysed, as were the concentrations of total H2O2, ascorbate and glutath...

Tomato (Lycopersicon esculentum) fruit carboxypeptidase active on N-carbobenzoxy Z-L-phenylalanine-L-alanine was found to constitute a family of isoforms whose abundance changed differentially during ripening. A specific polyclonal antibody against the fruit carboxypeptidase was raised in rabbits and used to purify and identify the protein. The data from immunoaffinity chromatography, immunoinh...

We have analyzed DNA methylation of plastid DNA from fully ripened red fruits, green mature fruits, and green leaves of tomato (Lycopersicon esculentum var. Firstmore). Essentially identical restriction profiles were obtained between chromoplast and chloroplast DNAs by EcoRI digestion. BstNI/EcoRII and HpaII/MspI are pairs of isoschizomers that can discriminate between methylated and unmethylat...

The codons for the amino acid residues making up the proposed ATP-binding sites of the maize (Zea mays L.) endoplasmic reticulum and tomato (Lycopersicon esculentum) cytoplasmic Stress70 proteins were deleted from their respective cDNAs. The deletions had little effect on the predicted secondary structure characteristics of the encoded proteins. Both wild-type and mutant proteins were expressed...

Levels of endogenous abscisic acid (ABA) in wild type were not required for the synthesis of heat shock proteins in detached leaves of tomato (Lycopersicon esculentum Mill., cv Ailsa Craig). Heat-induced alterations in gene expression were the same in the ABA-deficient mutant of tomato, flacca, and the wild type. Heat tolerance of the mutant was marginally less that the wild type, and in contra...

The aromas of fruits, vegetables, and flowers are mixtures of volatile metabolites, often present in parts per billion levels or less. We show here that tomato (Lycopersicon esculentum Mill.) plants transgenic for a heterologous Clarkia breweri S-linalool synthase (LIS) gene, under the control of the tomato late-ripening-specific E8 promoter, synthesize and accumulate S-linalool and 8-hydroxyli...

The reproduction of single egg-mass isolates of Meloidogyne javanica from Crete that differed in virulence were compared on tomato (Lycopersicon esculentum) genotypes homozygous or heterozygous for the Mi gene. The reproduction of three isolates with partial virulence was much greater on tomato genotypes heterozygous for the Mi gene (cultivars Scala, Bermuda, and 7353) than on two homozygous ge...