DNA synthesis was investigated in human malignant mela noma cells. The DNA was extracted in a neutral nondenaturing buffer at 25°and characterized by agarose gel electrophoresis. Radioactive DNA pulse labeled for 10 to 30 min was released from the chromosomes in the form of double-stranded frag ments. The release of the fragments from the chromosomes is prevented by lysing the cells at 0°inst...

PAS: periodic acideSchiff INTRODUCTION Although Entamoeba histolytica, named for its notorious tissue-lysing facility, is a major cause of morbidity and mortality worldwide, cutaneous involvement is rare. Presentation in nonendemic countries is exceptionally rare. We present a case of amebiasis cutis presenting as a deeply invasive perianal ulceration. Delayed diagnosis, empiric use of broad-sp...

We present a novel micromachined polymer peripheral nerve interface that combines both electrodes and microfluidic channels in an adjustable cuff sized to interface with rat sciatic nerve. Parylene fluidic channels enable targeted delivery of lysing agents and neurotrophic factors to fascicle sites on the nerve surface, in order to induce axonal sprouting towards electrodes. This Lyse-andAttrac...

We demonstrate a technique for purification of nucleic acids from malaria parasites infecting human erythrocytes using isotachophoresis (ITP). We release nucleic acids from malaria-infected erythrocytes by lysing with heat and proteinase K for 10 min and immediately, thereafter, load sample onto a capillary device. We study the effect of temperature on lysis efficiency. We also implement pressu...

A tyrothricin concentration of 0.01 mg. per ml. causes rapid lysis of Staphylococcus aureus; this lyric action is not enhanced by phage. A concentration of 0.001 mg. per ml. of tyrothricin is likewise capable of lysing staphylococci, and the addition of phage does not appreciably alter the rate of lysis. A concentration of 0.0001 mg. per ml. of tyrothricin is unable to effect lysis though it en...

In this study, animal and human pathogen Escherichia coli strains were isolated from Balcalı domestic sewage water. The isolated strains were identified and 37 strains which are sensitive to phage were selected. The plasmid isolation of bacteria strains was done and plasmid profiles were observed by agarose gel electrophoresis. Except some strains, most of the E.coli strains had 300-10,000 bp s...

5-Fluorouracil and 5-fluorodeoxyuridine induce DNA lesions via two different mechanisms, one involving and the other not involving the incorporation of drug into DNA. The DNA lesions are detected by lysing cells in dilute alkali and then separating the DNA in agarose gel electrophoresis. We examine here the effect of dipyridamole, a nucleoside transport inhibitor, on the DNA lesions. We find th...

Histopathological examinations of synovial tissues, obtained in 393 operations, disclosed the presence of foreign body giant cells or synovial giant cells or both in 51 cases. The presence of the former could be related to the occurrence of material foreign to the location, whereas for the latter group fo coincidence of precipitated fibrin only was noted in a significant number of cases. The pa...

Structures retaining many of the morphological features of nuclei may be released by lysing human cells in a non-ionic detergent and 2 M NaCl. Such nucleoids contain all the nuclear DNA packaged within a flexible cage of RNA and protein. HeLa nucleoids have been spread at an air-water interface and viewed in the electron microscope. A tangled network of superhelical fibres surrounds the collaps...