E. Granda ([email protected]) and F. Valladares, Lab. Internacional de Cambio Global (LINC-Global), Depto de Biogeograf í a y Cambio Global, Museo Nacional de Ciencias Naturales, MNCN, CSIC, Serrano 115 Dpdo., ES-28006 Madrid, Spain. FV also at: Depto de Biolog í a y Geolog í a, ESCET, Univ. Rey Juan Carlos, Tulip á n s/n, ES-28933 M ó stoles, Spain. – C. Scoff oni and L. Sack, Dept of E...

T h i s r e p o r t d e s c r i b e s r e s e a r c h done a t t h e A r t i f i c i a l I n t e l l i g e n c e L a b o r a t o r y o f t h e Massac h u s e t t s I n s t i t u t e o f T e c h n o l o g y . S u p p o r t f o r t h e l a b o r a t o r y ' s a r t i f i c i a l i n t e l l i g e n c e r e s e a r c h i s p r o v i d e d i n p a r t b y t h e Advanced Research P r o j e c t s Age...

Maintaining a quality of life for residents in older, fragile neighborhoods is challenging when wild parties, parking problems, loud music and peeing in the neighborhood peony’s dominates the scene. Lincoln, Nebraska’s oldest, poorest neighborhoods have a high percentage of non-owner occupied residents who are 1825 years old, transitory and feel no real relationship to the area. When increased ...

lamin gene (Lmna) develop an EDMD-like syndrome soon after birth and die within 6 weeks of cardiovascular disease. A recent publication by Chen et al. (2012) reports the remarkable finding that downregulation of Sun1 expression in Lmnadeficient mice significantly extended their life span along with amelioration of cardiac pathology. Thus, LINC complexes could represent viable therapeutic target...

SUN proteins are the core components of LINC complexes that span across the nuclear envelope for nuclear positioning and migration. SUN proteins contain at least one predicted coiled-coil domain preceding the SUN domain. Here, we found that the two coiled-coil domains (CC1 and CC2) of SUN2 exhibit distinct oligomeric states. CC2 is a monomer in solution. The structure of the CC2-SUN monomer rev...

Repeated rounds of nuclear envelope (NE) rupture and repair have been observed in laminopathy and cancer cells and result in intermittent loss of nucleus compartmentalization. Currently, the causes of NE rupture are unclear. Here, we show that NE rupture in cancer cells relies on the assembly of contractile actin bundles that interact with the nucleus via the linker of nucleoskeleton and cytosk...

The linker of nucleoskeleton and cytoskeleton (LINC) complex, composed of outer and inner nuclear membrane Klarsicht, ANC-1, and Syne homology (KASH) and Sad1 and UNC-84 (SUN) proteins, respectively, connects the nucleus to cytoskeletal filaments and performs diverse functions including nuclear positioning, mechanotransduction, and meiotic chromosome movements. Recent studies have shed light on...

Communication between nucleus and cytoplasm extends past molecular exchange and critically includes mechanical wiring. Cytoskeleton and nucleoskeleton are connected via molecular tethers that span the nuclear envelope. Sad1, UNC84 (SUN)-domain proteins spanning the inner nuclear membrane and Klarsicht, ANC-1 and SYNE/Nesprin-1 and -2 Homology (KASH)-peptide bearing proteins residing in the oute...

Little is known how lincRNAs are involved in skeletal myogenesis. Here we describe the discovery and functional annotation of Linc-YY1, a novel lincRNA originating from the promoter of the transcription factor (TF) Yin Yang 1 (YY1). Starting from whole transcriptome shotgun sequencing (a.k.a. RNA-seq) data from muscle C2C12 cells, a series of bioinformatics analysis was applied towards the iden...