Abstract The ovary contains numerous macrophages, and macrophage depletion in mice leads to dysfunctional ovulation. Macrophage function the is presumed be related efferocytosis, however, absence of macrophages also disrupts follicular development corpus luteum development. Granulosa cells (GC) are critical ovarian follicle survival, we hypothesized that macrophage-GC crosstalk promoted GC func...