Mitochondrial dysfunction has been implicated in the dopaminergic neurodegeneration, which characterizes Parkinson’s disease (PD). The activities of mitochondrial complexes I and IV were found to be reduced in the brains of PD patients (n = 4) as compared to age-matched controls (n = 4). This is tested in SH-SY5Y cell lines, transformed Rho0 cells, and in normal and PD cybrid cell lines. Cybrid...

Mitochondrial dysfunction has been implicated in the dopaminergic neurodegeneration, which characterizes Parkinson’s disease (PD). The activities of mitochondrial complexes I and IV were found to be reduced in the brains of PD patients (n = 4) as compared to age-matched controls (n = 4). This is tested in SH-SY5Y cell lines, transformed Rho0 cells, and in normal and PD cybrid cell lines. Cybrid...

A method for preparation of highly active mammalian ribosomal subunits is described, which yields 60S subunits containing no more than 33% protein. It is suggested that the composition of these subunits corresponds closely to that of Escherichia coli 50S subunits. Data on the composition of bacterial and mammalian ribosomal subunits recovered from CsCl are given. It is shown that the commonly e...

Ca2+ channels are voltage-gated ion present in the membrane of most excitable cells. The nomenclature for Ca2+channels was proposed by [131] and approved NC-IUPHAR Subcommittee on [72]. Most form hetero-oligomeric complexes. α1 subunit is pore-forming provides binding site(s) practically all agonists antagonists. 10 cloned α1-subunits can be grouped into three families: (1) high-voltage activat...

The complexation of (3aR,7aR)-N-(3,5-bis(trifluoromethyl)phenyl)octahydro-2H-benzo[d]imidazol-2-imine (BTI), as a guest, to ethane-bridged bis(zinc octaethylporphyrin), bis(ZnOEP), host, has been studied by means ultraviolet-visible (UV-Vis) and circular dichroism (CD) absorption spectroscopies, single crystal X-ray diffraction, computational simulation. formation 1:2 host-guest complex was est...

Higher education institutions are pressured to use data for university rankings, accreditation, decision making, and technical support of students faculty. However, these organizations also experience barriers resistance such data-driven aspirations. Rather than simply focusing on the whole organization, this research attends variations in organizational subunits asks what processes help hinder...

Macauba palm (Acrocomia aculeata L.) fruits are emerging sources to produce high-quality oils from pulp and kernels. Yet, the protein-rich press cake kernels remains underutilized, mainly due lacking knowledge of protein composition properties. Therefore, our aim was characterize main fractions The globulins were major fraction (58.5% storage proteins), which further separated into 11 S 7 globu...

The 26S proteasome is the largest and most complicated protease known, changes to assembly or function contribute numerous human diseases. Assembly of from its ~66 individual polypeptide subunits a highly orchestrated process requiring concerted actions both intrinsic elements subunits, as well assistance by extrinsic, dedicated chaperones. With advent near-atomic resolution cryo-electron micro...

Adenylyl cyclases (ACs) play a key role in many signaling cascades. ACs catalyze the production of cyclic AMP from ATP and this function is stimulated or inhibited by binding their cognate stimulatory inhibitory Gα subunits, respectively. Here we used simulation tools to uncover molecular subcellular mechanisms AC function, with focus on AC5 isoform, extensively studied experimentally. First, q...