The poor efficiency of microfluidic single cell trapping is currently restricting the full potential of state-of-the-art single cell analyses. Using fluid dynamics simulations in combination with particle image velocimetry to systematically optimize trap architectures, we present a microfluidic chip with enhanced single cell trapping and on-chip culture performance. Upon optimization of trap ge...

M U C H of our knowledge concerning the molecular processes of the cell has come from studies on unicellular organisms, as a result of several properties which suit them to experimentation. In contrast, the complex life cycles and multicellular organization of differentiated higher organisms have put experiments on an entirely different time-scale and have made the biochemical analysis of any s...

The use of space environment to carry out life science research is great significance. However, there are limitations in complex manipulation, models based on mammalian and 2-dimensional cell culture experiments, etc. Microfluidic chips possess the characteristics integrating relevant functions traditional laboratories into micron-scale chips, with low energy consumption, high throughput, autom...

Sonoporation, which is the perforation of cell membranes using ultrasound and microbubble, has been shown to facilitate liposome-mediated delivery nucleotides in mammalian cells. The effect not yet tested non-vertebrate species such as fish. Since adenoviral vector associated with tumorigenicity electroporation affected by ions content culture water, sonoporation may be an ideal means enhancing...

objective(s): irinotecan is a potent anti-cancer drug from camptothecin group which inhibits topoisomerase i. recently, biodegradable and biocompatible polymers such as poly lactide-co-glycolides (plga) have been considered for the preparation of nanoparticles (nps). materials and methods: in this study, irinotecan loaded plga nps were fabricated by an emulsification/solvent diffusion method to...

Our primary focus is enabling and accelerating biological drug manufacture through the development of costeffective technologies that facilitate rapid bioprocess development and improve manufacturing “bang-for-thebuck”. Using data mining and cell-based screens we have designed the RegocelTM small molecule yield enhancers for a number of mammalian cell platforms used in biomanufacturing such as ...

Background Melatonin (N-acetyl-5- methoxytryptamine) is mainly synthesized and secreted in the pineal gland, ovary, testes, bone marrow, retina and lens in mammalian species. It is involved in the detoxification of ROS and protects embryos from oxidative damage. Melatonin acts as a potential free radical scavenger, including peroxyl radical and hydroxyl radical. In addition, it can stimulate th...

Background: Mammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. However, it is still unclear about the crucial transcriptional network and key hub genes that regulate the proceeding of preimplantation embryos. Materials and Methods: Through single-cell RNAsequencing (RNA-seq) of both human and mouse preimplantation embryos, ...

PURPOSE Fully mature neurons of central nervous system origin generally are considered unable to survive for extended periods of time in simple culture conditions. The authors report that adult and aged human, porcine, and rodent retinal neurons, including rod and cone photoreceptors, constitute an exception to this idea. METHODS Cells were dissociated from human postmortem retinas, adult mam...